ArticleDiscovery medicine
Cholesterol crystals induce inflammatory cytokines expression in a human retinal pigment epithelium cell line by activating the NF-κB pathway.
Article in Discovery medicine. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The trial behind it
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Who cites it
12 citing papers in PubMed, 16 citations in OpenAlex.
- Cholesterol crystals in the pathogenesis of atherosclerosis.Nature reviews. Cardiology · 2025Review
- The impact of oxygen content onFrontiers in microbiology · 2024Article
- A causal association between lipid-lowering medications and rotator cuff syndrome: a drug-targeted mendelian randomization study.Frontiers in genetics · 2024Article
- Progress in studying the impact of hyperlipidemia and statins on rotator cuff injury and repair.Frontiers in public health · 2023Review
- The complement C3a-C3aR and C5a-C5aR pathways promote viability and inflammation of human retinal pigment epithelium cells by targeting NF-κB signaling.Experimental and therapeutic medicine · 2022Article
- Formation and Cellular Impact of Cholesterol Crystals in Health and Disease.Advanced biology · 2021Review
- Protective role and molecular mechanism of action of Nesfatin-1 against high glucose-induced inflammation, oxidative stress and apoptosis in retinal epithelial cells.Experimental and therapeutic medicine · 2021Article
- NOD-like Receptors in the Eye: Uncovering Its Role in Diabetic Retinopathy.International journal of molecular sciences · 2020Review
- Ultramorphological analysis of plaque advancement and cholesterol crystal formation in Ldlr knockout mouse atherosclerosis.Atherosclerosis · 2019Article
- Atorvastatin Promotes Phagocytosis and Attenuates Pro-Inflammatory Response in Human Retinal Pigment Epithelial Cells.Scientific reports · 2017Article
- Article
- Mitochondrial DNA has a pro-inflammatory role in AMD.Biochimica et biophysica acta · 2015Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
purposeTo investigate the expression of inflammatory cytokines in ARPE-19 cells after stimulation with cholesterol crystals.
methodsAPRE-19 cells were cultured, primed with IL-1α, and treated with cholesterol crystals under different concentrations. Inflammatory cytokines (mature-IL-1β, IL-6, and IL-8) in supernatant and inflammatory cytokines (pro-IL-1β, IL-18) in cell lysate were detected by western blot. The NF-κB pathway inhibitor BAY 11-7082 was used to determine the pathway of cytokine expression.
resultsCholesterol crystals did not induce the nucleotide-binding domain leucine-rich repeat containing family, pyrin domain containing 3 (NLRP3) inflammasome, but did increase pro-IL-1β expression in ARPE-19 cells. Cholesterol crystals increased pro-IL-1β expression by activating the NF-κB pathway. Cholesterol crystal activation of the NF-κB pathway also leads to increased IL-6 and IL-8 expression.
conclusionCholesterol crystals can induce inflammatory cytokine expression in ARPE-19 cells by activating the NF-κB pathway.
Indexed as
Identifiers
25091484PMC5330255W2395308401What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.