ArticleBMC genomics2014
Genome-wide annotation and functional identification of aphid GLUT-like sugar transporters.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 37 citations in OpenAlex.
- Genome-wide identification, characterization, and expression analyses of the solute carrier 2 (SLC2) gene family in fall armyworm (Spodoptera frugiperda).BMC genomics · 2026Article
- Gut bacteria are essential for development of an invasive bark beetle by regulating glucose transport.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Genome-Wide Identification and Expression Pattern of Sugar Transporter Genes in the Brown Planthopper,Insects · 2024Article
- Sugar transporters enable a leaf beetle to accumulate plant defense compounds.Nature communications · 2021Article
- Genome-Wide Characterization and Expression Profiling of Sugar Transporter Family in the Whitefly,Frontiers in physiology · 2017Article
- Pea aphid infestation induces changes in flavonoids, antioxidative defence, soluble sugars and sugar transporter expression in leaves of pea seedlings.Protoplasma · 2016Article
- The multi-tasking gut epithelium of insects.Insect biochemistry and molecular biology · 2015Review
- Silencing a sugar transporter gene reduces growth and fecundity in the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae).Scientific reports · 2015Article
- The molecular correlates of organ loss: the case of insect Malpighian tubules.Biology letters · 2015Article
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2 authors at 1 institution in 1 country.
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Abstract
backgroundPhloem feeding insects, such as aphids, feed almost continuously on plant phloem sap, a liquid diet that contains high concentrations of sucrose (a disaccharide comprising of glucose and fructose). To access the available carbon, aphids hydrolyze sucrose in the gut lumen and transport its constituent monosaccharides, glucose and fructose. Although sugar transport plays a critical role in aphid nutrition, the molecular basis of sugar transport in aphids, and more generally across all insects, remains poorly characterized. Here, using the latest release of the pea aphid, Acyrthosiphon pisum, genome we provide an updated gene annotation and expression profile of putative sugar transporters. Finally, gut expressed sugar transporters are functionally expressed in yeast and screened for glucose and fructose transport activity.
resultsIn this study, using a de novo approach, we identified 19 sugar porter (SP) family transporters in the A. pisum genome. Gene expression analysis, based on 214, 834 A. pisum expressed sequence tags, supports 17 sugar porter family transporters being actively expressed in adult female aphids. Further analysis, using quantitative PCR identifies 4 transporters, A. pisum sugar transporter 1, 3, 4 and 9 (ApST1, ApST3, ApST4 and ApST9) as highly expressed and/or enriched in gut tissue. When expressed in a Saccharomyces cerevisiae hexose transporter deletion mutant (strain EBY.VW4000), only ApST3 (previously characterized) and ApST4 (reported here) transport glucose and fructose resulting in functional rescue of the yeast mutant. Here we characterize ApST4, a 491 amino acid protein, with 12 predicted transmembrane regions, as a facilitative glucose/fructose transporter. Finally, phylogenetic reconstruction reveals that ApST4, and related, as yet uncharacterized insect transporters are phylogenetically closely related to human GLUT (SLC2A) class I facilitative glucose/fructose transporters.
conclusionsThe gut enhanced expression of ApST4, and the transport specificity of its product is consistent with ApST4 functioning as a gut glucose/fructose transporter. Here, we hypothesize that both ApST3 (reported previously) and ApST4 (reported here) function at the gut interface to import glucose and fructose from the gut lumen.
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