Evidence map›Paper›PMID 25091190›Full record

ArticleOtolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery2014

Matrix-metalloproteinases in head and neck carcinoma-cancer genome atlas analysis and fluorescence imaging in mice.

Samantha J Hauff, Sharat C Raju, Ryan K Orosco, Andrew M Gross, Julio A Diaz-Perez, Elamprakash Savariar, Nadia Nashi, Jonathan Hasselman, Michael Whitney, Jeffrey N Myers and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.8field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 31 citations in OpenAlex.

  1. Review
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  12. SensitiveTheranostics · 2017
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  14. Observational
  15. FRET-trackable biodegradable HPMA copolymer-epirubicin conjugates for ovarian carcinoma therapy.Journal of controlled release : official journal of the Controlled Release Society · 2015
    Article
  16. Article
  17. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 1 country.

Samantha J HauffDivision of Head and Neck Surgery, University of California, San Diego, California, USA.
Sharat C RajuDivision of Head and Neck Surgery, University of California, San Diego, California, USA.
Ryan K OroscoDivision of Head and Neck Surgery, University of California, San Diego, California, USA.
Andrew M GrossBioinformatics and Systems Biology Program, University of California, San Diego, California, USA.
Julio A Diaz-PerezDivision of Head and Neck Surgery, University of California, San Diego, California, USA.
Elamprakash SavariarDepartment of Pharmacology, University of California, San Diego, California, USA.
Nadia NashiDivision of Head and Neck Surgery, University of California, San Diego, California, USA.
Jonathan HasselmanDivision of Head and Neck Surgery, University of California, San Diego, California, USA.
Michael WhitneyDepartment of Pharmacology, University of California, San Diego, California, USA.
Jeffrey N MyersDepartment of Head and Neck Surgery, University of Texas MD Anderson Cancer Center, Houston, Texas, USA.
Scott M LippmanMoores Cancer Center, University of California, San Diego, California, USA.
Roger Y TsienDepartment of Pharmacology, University of California, San Diego, California, USA Howard Hughes Medical Institute, San Diego, California, USA.
Trey IdekerBioinformatics and Systems Biology Program, University of California, San Diego, California, USA Division of Medical Genetics, University of California, San Diego, California, USA.
Quyen T NguyenDivision of Head and Neck Surgery, University of California, San Diego, California, USA Moores Cancer Center, University of California, San Diego, California, USA quyennguyen@ucsd.edu.
University of California, San Diego · USHoward Hughes Medical Institute · USThe University of Texas MD Anderson Cancer Center · US

Funding

Targeting the p53 pathway for reversal of oral premalignancyP50CA097007 · NCI · UNIVERSITY OF TX MD ANDERSON CAN CTR · PI MYERS, JEFFREY NICHOLAS · 2002 to 2012
$26.1M
Injectable reporters to image tumors and guide resectionR01CA158448 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI NGUYEN, QUYEN · 2011 to 2015
$3.1M
OTOLARYNGOLOGY TRAINING IN IMMUN., VIROL. &MOLEC. BIOL.T32DC000028 · NIDCD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI RYAN, ALLEN F. · 1989 to 2020
$2.1M
Testing Fluorescently Labeled Probes for Nerve Imaging during SurgeryR01EB014929 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI NGUYEN, QUYEN · 2012 to 2016
$1.7M
San Diego Clinical and Translational Research InstituteTL1RR031979 · NCRR · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI FIRESTEIN, GARY S · 2010 to 2011
$885k
NCI NIH HHS P50 CA097007NCI NIH HHS R01 CA158448NCRR NIH HHS RR031979NCRR NIH HHS TL1 RR031979NIBIB NIH HHS R01 EB014929NIDCD NIH HHS T32 DC000028Wellcome Trust
6 · The paper itself

Abstract

objective(1) Obtain matrix-metalloproteinase (MMP) expression profiles for head and neck squamous cell carcinoma (HNSCC) specimens from the Cancer Genomic Atlas (TCGA). (2) Demonstrate HNSCC imaging using MMP-cleavable, fluorescently labeled ratiometric activatable cell-penetrating peptide (RACPP). STUDY

designRetrospective human cohort study; prospective animal study.

settingTranslational research laboratory. SUBJECTS AND

methodsPatient clinical data and mRNA expression levels of MMP genes were downloaded from TCGA data portal. RACPP provides complementary ratiometric fluorescent contrast (increased Cy5 and decreased Cy7 intensities) when cleaved by MMP2/9. HNSCC-tumor bearing mice were imaged in vivo after RACPP injection. Histology was evaluated by a pathologist blinded to experimental conditions. Zymography confirmed MMP-2/9 activity in xenografts. RACPP was applied to homogenized human HNSCC specimens, and ratiometric fluorescent signal was measured on a microplate reader for ex vivo analysis.

resultsExpression of multiple MMPs including MMP2/9 is greater in patient HNSCC tumors than matched control tissue. In patients with human papilloma virus positive (HPV+) tumors, higher MMP2 and MMP14 expression correlates with worse 5-year survival. Orthotopic tongue HNSCC xenografts showed excellent ratiometric fluorescent labeling with MMP2/9-cleavable RACPP (sensitivity = 95.4%, specificity = 95.0%). Fluorescence ratios were greater in areas of higher tumor burden (P < .03), which is useful for intraoperative margin assessment. Ex vivo, human HNSCC specimens showed greater cleavage of RACPP when compared to control tissue (P = .009).

conclusionsHuman HNSCC tumors show increased mRNA expression of multiple MMPs including MMP2/9. We used RACPP, a ratiometric fluorescence assay of MMP2/9 activity, to show improved occult tumor identification and margin clearance. Ex vivo assays using RACPP in biopsy specimens may identify patients who will benefit from intraoperative RACPP use.

Indexed as

AnimalsCarcinoma, Squamous CellCell Line, TumorCell-Penetrating PeptidesDisease Models, AnimalHead and Neck NeoplasmsHumansMatrix MetalloproteinasesMiceNeoplasm ProteinsNeoplasm TransplantationOptical ImagingPapillomaviridaeRetrospective StudiesRNA, MessengerSquamous Cell Carcinoma of Head and NeckCell-Penetrating PeptidesMatrix MetalloproteinasesNeoplasm ProteinsRNA, Messengerfluorescence imaginghead and neck squamous cell carcinomahuman papilloma virusthe Cancer Genomic Atlas

Identifiers

PMID25091190
PMCPMC4469264
OpenAlexW2056363107

What OpenQuestion holds

Textmetadata
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.