ArticleOptics express2014
Designing the focal plane spacing for multifocal plane microscopy.
Article in Optics express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Simultaneous particle tracking, phase retrieval and point spread function reconstruction.bioRxiv : the preprint server for biology · 2025Article
- Joint estimation of point spread function and molecule positions in SMLM informed from multiple planes.Biomedical optics express · 2025Article
- Simultaneous Multifocal Plane Fourier Ptychographic Microscopy Utilizing a Standard RGB Camera.Sensors (Basel, Switzerland) · 2024Article
- Recent Advances in Single-Molecule Tracking and Imaging Techniques.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Instantaneous 4D micro-particle image velocimetry (µPIV) via multifocal microscopy (MUM).Scientific reports · 2022Article
- Fundamental precision bounds for three-dimensional optical localization microscopy using self-interference digital holography.Biomedical optics express · 2021Article
- Effect of Pixelation on the Parameter Estimation of Single Molecule Trajectories.IEEE transactions on computational imaging · 2021Article
- Fidelity test for through-focus or volumetric type of optical imaging methods.Optics express · 2018Article
- Through-focus or volumetric type of optical imaging methods: a review.Journal of biomedical optics · 2018Review
- Three-Dimensional Localization of Single Molecules for Super-Resolution Imaging and Single-Particle Tracking.Chemical reviews · 2017Review
- Fisher information theory for parameter estimation in single molecule microscopy: tutorial.Journal of the Optical Society of America. A, Optics, image science, and vision · 2016Article
- Deciphering the Structure and Function of Nuclear Pores Using Single-Molecule Fluorescence Approaches.Journal of molecular biology · 2016Review
- New results on the single molecule localization problem in two and three dimensions.Proceedings of SPIE--the International Society for Optical Engineering · 2015Article
- Determination of localization accuracy based on experimentally acquired image sets: applications to single molecule microscopy.Optics express · 2015Article
- 3D multifocus astigmatism and compressed sensing (3D MACS) based superresolution reconstruction.Biomedical optics express · 2015Article
- An information-theoretic approach to designing the plane spacing for multifocal plane microscopy.Proceedings of SPIE--the International Society for Optical Engineering · 2015Article
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Authors and funding
7 authors.
Funding
Abstract
Multifocal plane microscopy (MUM) has made it possible to study subcellular dynamics in 3D at high temporal and spatial resolution by simultaneously imaging distinct planes within the specimen. MUM allows high accuracy localization of a point source along the z-axis since it overcomes the depth discrimination problem of conventional single plane microscopy. An important question in MUM experiments is how the number of focal planes and their spacings should be chosen to achieve the best possible localization accuracy along the z-axis. Here, we propose approaches based on the Fisher information matrix and report spacing scenarios called strong coupling and weak coupling which yield an appropriate 3D localization accuracy. We examine the effect of numerical aperture, magnification, photon count, emission wavelength and extraneous noise on the spacing scenarios. In addition, we investigate the effect of changing the number of focal planes on the 3D localization accuracy. We also introduce a new software package that provides a user-friendly framework to find appropriate plane spacings for a MUM setup. These developments should assist in optimizing MUM experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.