ArticleJournal of chromatography. B, Analytical technologies in the biomedical and life sciences2014
Comprehensive quantitative analysis of purines and pyrimidines in the human malaria parasite using ion-pairing ultra-performance liquid chromatography-mass spectrometry.
Article in Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Distinct evolution of type I glutamine synthetase in Plasmodium and its species-specific requirement.Nature communications · 2023Article
- Metabolic Profiling of Water-Soluble Compounds from the Extracts of Dark Septate Endophytic Fungi (DSE) Isolated from Scots Pine (Molecules (Basel, Switzerland) · 2019Article
- Nucleotide Analysis in Korean Dairy Products Using High- Performance Liquid Chromatography with Diode Array Detector.Food science of animal resources · 2019Article
- T cell activation triggers reversible inosine-5'-monophosphate dehydrogenase assembly.Journal of cell science · 2018Article
- Biological Studies and Target Engagement of the 2- C-Methyl-d-Erythritol 4-Phosphate Cytidylyltransferase (IspD)-Targeting Antimalarial Agent (1 R,3 S)-MMV008138 and Analogs.ACS infectious diseases · 2018Article
- Reconstituted IMPDH polymers accommodate both catalytically active and inactive conformations.Molecular biology of the cell · 2017Article
- Article
- Attomole quantification and global profile of RNA modifications: Epitranscriptome of human neural stem cells.Nucleic acids research · 2016Article
- Cyclic di-GMP modulates gene expression in Lyme disease spirochetes at the tick-mammal interface to promote spirochete survival during the blood meal and tick-to-mammal transmission.Infection and immunity · 2015Article
- Purine import into malaria parasites as a target for antimalarial drug development.Annals of the New York Academy of Sciences · 2015Review
- Isoprenoid precursor biosynthesis is the essential metabolic role of the apicoplast during gametocytogenesis in Plasmodium falciparum.Eukaryotic cell · 2015Article
- The identification and characterization of non-coding and coding RNAs and their modified nucleosides by mass spectrometry.RNA biology · 2014Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Targeted metabolite profiling has aided in the understanding of a variety of biological processes in the malaria parasite as well as in drug discovery. A fast and sensitive analytical method, based on ion-pairing reversed phase ultra-high performance liquid chromatography tandem mass spectrometry (IP-RP-UPLC-MS/MS), was optimized for the simultaneous analysis of intracellular levels of 35 purine and pyrimidine nucleobases, nucleosides, and nucleotides. This analytical method allows for chromatographic separation of highly polar metabolites using reverse phase chemistry within 15 min. The analytical performance of the method was evaluated and successfully applied to the quantification of purines and pyrimidines in Plasmodium falciparum and its host cell, the human erythrocyte. In addition, this method can be customized to include other related metabolites such as NADPH and NADP, among others.
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Registered trials
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