ArticlePloS one2014
Microbial population analysis of the salivary glands of ticks; a possible strategy for the surveillance of bacterial pathogens.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 132 citations in OpenAlex.
- Tick Microbiome and Its Role in Emerging Zoonotic Diseases and Transmissibility.Microorganisms · 2026Review
- High throughput sequencing in the diagnosis and surveillance of tick-borne diseases: A narrative review.One health (Amsterdam, Netherlands) · 2026Review
- A human-associatediScience · 2026Article
- A review of filarial nematodes parasitizing tick vectors: unraveling global patterns in species diversity, host associations, and interactions with tick-borne pathogens.Parasites & vectors · 2025Review
- Genetic and genomic variability ofISME communications · 2025Article
- Metagenome reveals the midgut microbial community of Haemaphysalis qinghaiensis ticks collected from yaks and Tibetan sheep.Parasites & vectors · 2024Article
- Molecular screening and genetic diversity of tick-borne pathogens associated with dogs and livestock ticks in Egypt.PLoS neglected tropical diseases · 2024Article
- Impact of endosymbionts on tick physiology and fitness.Parasitology · 2023Review
- Investigation of vertical and horizontal transmission of Spiroplasma in ticks under laboratory conditions.Scientific reports · 2023Article
- The dynamics of the microbiome in Ixodidae are shaped by tick ontogeny and pathogens in Sarawak, Malaysian Borneo.Microbial genomics · 2023Article
- Article
- Wild Hedgehogs and Their Parasitic Ticks Coinfected with Multiple Tick-Borne Pathogens in Jiangsu Province, Eastern China.Microbiology spectrum · 2022Article
- Where's the Beef? Understanding Allergic Responses to Red Meat in Alpha-Gal Syndrome.Journal of immunology (Baltimore, Md. : 1950) · 2022Review
- Recently EvolvedFrontiers in cellular and infection microbiology · 2022Article
- What do we know about the microbiome ofFrontiers in cellular and infection microbiology · 2022Review
- Species-Level Profiling ofGenetic testing and molecular biomarkers · 2021Article
- Molecular Detection and Genotyping ofPathogens (Basel, Switzerland) · 2021Article
- An integrated overview of the bacterial flora composition of Hyalomma anatolicum, the main vector of CCHF.PLoS neglected tropical diseases · 2021Article
- Identification of tick-borne pathogens by metagenomic next-generation sequencing in Dermacentor nuttalli and Ixodes persulcatus in Inner Mongolia, China.Parasites & vectors · 2021Article
- Applications of Blocker Nucleic Acids and Non-Metazoan PCR Improves the Discovery of the Eukaryotic Microbiome in Ticks.Microorganisms · 2021Article
2 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ticks are one of the most important blood-sucking vectors for infectious microorganisms in humans and animals. When feeding they inject saliva, containing microbes, into the host to facilitate the uptake of blood. An understanding of the microbial populations within their salivary glands would provide a valuable insight when evaluating the vectorial capacity of ticks. Three tick species (Ixodes ovatus, I. persulcatus and Haemaphysalis flava) were collected in Shizuoka Prefecture of Japan between 2008 and 2011. Each tick was dissected and the salivary glands removed. Bacterial communities in each salivary gland were characterized by 16S amplicon pyrosequencing using a 454 GS-Junior Next Generation Sequencer. The Ribosomal Database Project (RDP) Classifier was used to classify sequence reads at the genus level. The composition of the microbial populations of each tick species were assessed by principal component analysis (PCA) using the Metagenomics RAST (MG-RAST) metagenomic analysis tool. Rickettsia-specific PCR was used for the characterization of rickettsial species. Almost full length of 16S rDNA was amplified in order to characterize unclassified bacterial sequences obtained in I. persulcatus female samples. The numbers of bacterial genera identified for the tick species were 71 (I. ovatus), 127 (I. persulcatus) and 59 (H. flava). Eighteen bacterial genera were commonly detected in all tick species. The predominant bacterial genus observed in all tick species was Coxiella. Spiroplasma was detected in Ixodes, and not in H. flava. PCA revealed that microbial populations in tick salivary glands were different between tick species, indicating that host specificities may play an important role in determining the microbial complement. Four female I. persulcatus samples contained a high abundance of several sequences belonging to Alphaproteobacteria symbionts. This study revealed the microbial populations within the salivary glands of three species of ticks, and the results will contribute to the knowledge and prediction of emerging tick-borne diseases.
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