ArticleACS chemical biology2014
A double-hotdog with a new trick: structure and mechanism of the trans-acyltransferase polyketide synthase enoyl-isomerase.
Article in ACS chemical biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
- Molecular Decoration and Unconventional Double Bond Migration in Irumamycin Biosynthesis.Antibiotics (Basel, Switzerland) · 2024Article
- Diene incorporation by a dehydratase domain variant in modular polyketide synthases.Nature chemical biology · 2022Article
- Article
- The structure of a polyketide synthase bimodule core.Science advances · 2022Article
- The Sandarazols are Cryptic and Structurally Unique Plasmid-Encoded Toxins from a Rare Myxobacterium*.Angewandte Chemie (International ed. in English) · 2021Article
- Site directed mutagenesis as a precision tool to enable synthetic biology with engineered modular polyketide synthases.Synthetic and systems biotechnology · 2020Review
- Mining Symbionts of a Spider-Transmitted Fungus Illuminates Uncharted Biosynthetic Pathways to Cytotoxic Benzolactones.Angewandte Chemie (International ed. in English) · 2020Article
- Article
- Activation of silent biosynthetic gene clusters using transcription factor decoys.Nature chemical biology · 2019Article
- Molecular Basis for Olefin Rearrangement in the Gephyronic Acid Polyketide Synthase.ACS chemical biology · 2018Article
- The modules of trans-acyltransferase assembly lines redefined with a central acyl carrier protein.Proteins · 2018Article
- Structural and Functional Studies of a Pyran Synthase Domain from a trans-Acyltransferase Assembly Line.ACS chemical biology · 2018Article
- New Alpiniamides FromFrontiers in microbiology · 2018Article
- Article
- Structural and Functional Trends in Dehydrating Bimodules from trans-Acyltransferase Polyketide Synthases.Structure (London, England : 1993) · 2017Article
- The Uncommon Enzymology of Cis-Acyltransferase Assembly Lines.Chemical reviews · 2017Review
- Polyketide stereocontrol: a study in chemical biology.Beilstein journal of organic chemistry · 2017Review
- Vinylogous Dehydration by a Polyketide Dehydratase Domain in Curacin Biosynthesis.Journal of the American Chemical Society · 2016Article
- Functional Analysis of the Fusarielin Biosynthetic Gene Cluster.Molecules (Basel, Switzerland) · 2016Article
- Harnessing natural product assembly lines: structure, promiscuity, and engineering.Journal of industrial microbiology & biotechnology · 2016Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Many polyketide natural products exhibit invaluable medicinal properties, yet much remains to be understood regarding the machinery responsible for their biosynthesis. The recently discovered trans-acyltransferase polyketide synthases employ processing enzymes that catalyze modifications unique from those of the classical cis-acyltransferase polyketide synthases. The enoyl-isomerase domains of these megasynthases shift double bonds and are well-represented by an enzyme that helps forge the triene system within the antibiotic produced by the prototypical bacillaene synthase. This first crystal structure of an enoyl-isomerase, at 1.73 Å resolution, not only revealed relationships between this class of enzymes and dehydratases but also guided an investigation into the mechanism of double bond migration. The catalytic histidine, positioned differently from that of dehydratases, was demonstrated to independently shuttle a proton between the γ- and α-positions of the intermediate. This unprecedented mechanism highlights the catalytic diversity of divergent enzymes within trans-acyltransferase polyketide synthases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.