ArticlePloS one2014
High resolution scanning electron microscopy of cells using dielectrophoresis.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
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- Three-dimensional imaging of biological cells using surface plasmon coupled emission.Journal of biomedical optics · 2022Article
- Dielectrophoresis Prototypic Polystyrene Particle Synchronization toward Alive Keratinocyte Cells for Rapid Chronic Wound Healing.Sensors (Basel, Switzerland) · 2021Article
- Novel Methods to Manipulate Autolysis in Sparkling Wine: Effects on Yeast.Molecules (Basel, Switzerland) · 2021Article
- Analytical Guidelines for Designing Curvature-Induced Dielectrophoretic Particle Manipulation Systems.Micromachines · 2020Article
- In-situ preparation of plant samples in ESEM for energy dispersive x-ray microanalysis and repetitive observation in SEM and ESEM.Scientific reports · 2019Article
- A review of polystyrene bead manipulation by dielectrophoresis.RSC advances · 2019Review
- A low voltage nanopipette dielectrophoretic device for rapid entrapment of nanoparticles and exosomes extracted from plasma of healthy donors.Scientific reports · 2018Article
- Three-Dimensional Reservoir-Based Dielectrophoresis (rDEP) for Enhanced Particle Enrichment.Micromachines · 2018Article
- Magnetic Force-Based Microfluidic Techniques for Cellular and Tissue Bioengineering.Frontiers in bioengineering and biotechnology · 2018Review
- Review: Microbial analysis in dielectrophoretic microfluidic systems.Analytica chimica acta · 2017Review
- Rapid isolation of blood plasma using a cascaded inertial microfluidic device.Biomicrofluidics · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ultrastructural analysis of cells can reveal valuable information about their morphological, physiological, and biochemical characteristics. Scanning electron microscopy (SEM) has been widely used to provide high-resolution images from the surface of biological samples. However, samples need to be dehydrated and coated with conductive materials for SEM imaging. Besides, immobilizing non-adherent cells during processing and analysis is challenging and requires complex fixation protocols. In this work, we developed a novel dielectrophoresis based microfluidic platform for interfacing non-adherent cells with high-resolution SEM at low vacuum mode. The system enables rapid immobilization and dehydration of samples without deposition of chemical residues over the cell surface. Moreover, it enables the on-chip chemical stimulation and fixation of immobilized cells with minimum dislodgement. These advantages were demonstrated for comparing the morphological changes of non-budding and budding yeast cells following Lyticase treatment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.