ArticleThe Journal of urology2015
High quality RNA in semen and sperm: isolation, analysis and potential application in clinical testing.
Article in The Journal of urology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Who cites it
23 citing papers in PubMed, 41 citations in OpenAlex.
- Clinical Implications of Paternal Age in Assisted Reproduction: Integrating Sperm Epigenetic Evidence.Journal of clinical medicine · 2026Review
- Development and Optimization of an RNA-Isolating Protocol for Mammalian Spermatozoa.International journal of molecular sciences · 2025Article
- The use of environmental RNA for inferring fish spawning behavior.Scientific reports · 2025Article
- A narrative review of sperm selection technology for assisted reproduction techniques.Translational andrology and urology · 2024Review
- Small RNAs: an ideal choice as sperm quality biomarkers.Frontiers in reproductive health · 2024Article
- Expression of estrogen receptors, PELP1, and SRC in human spermatozoa and their associations with semen quality.Human cell · 2023Article
- A comparative assessment ofInternational journal of reproductive biomedicine · 2022Article
- Sperm Transcriptome Analysis Accurately Reveals Male Fertility Potential in Livestock.Animals : an open access journal from MDPI · 2022Review
- Alteration of RNA modification signature in human sperm correlates with sperm motility.Molecular human reproduction · 2022Article
- Paternal diet induces transgenerational epigenetic inheritance of DNA methylation signatures and phenotypes in sheep model.PNAS nexus · 2022Article
- Comparison betweenInternational journal of fertility & sterility · 2022Article
- The ribosome inhibitor chloramphenicol induces motility deficits in human spermatozoa: A proteomic approach identifies potentially involved proteins.Frontiers in cell and developmental biology · 2022Article
- Characterization of Breed Specific Differences in Spermatozoal Transcriptomes of Sheep in Australia.Genes · 2021Article
- Roles for the RNA polymerase III regulator MAFR-1 in regulating sperm quality in Caenorhabditis elegans.Scientific reports · 2020Article
- Optimization of sperm RNA processing for developmental research.Scientific reports · 2020Article
- Human sperm displays rapid responses to diet.PLoS biology · 2019Article
- Optimizing PCR Detection of Zika Virus from Various Body Fluids.The American journal of tropical medicine and hygiene · 2019Article
- System analysis of teratozoospermia mRNA profile based on integrated bioinformatics tools.Molecular medicine reports · 2018Article
- High-quality human and rat spermatozoal RNA isolation for functional genomic studies.Andrology · 2018Article
- Ion channel gene expressions in infertile men: A case-control study.International journal of reproductive biomedicine · 2017Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
purposeMale infertility is a complex health condition. To our knowledge there are no molecular biomarkers of male infertility. Sperm RNA is a potential biomarker for detecting sperm abnormalities and viability at infertility clinics. However, RNA use is hindered by its inconsistent quantity, quality, multiple cell types in semen and condensed sperm structure. MATERIALS AND
methodsWe tested the usefulness of high quality RNA isolated from mature sperm and whole semen by our protocol, which reduces RNA degradation by maintaining semen and protocol components at 37 C and decreasing processing time. We isolated RNA from 83 whole semen samples, 18 samples of motile sperm prepared by the swim-up protocol and 18 of sperm prepared by the standard Percoll gradient method.
resultsElectrophoretic and spectral analysis of RNA revealed high quality 18S and 28S rRNAs in 71 of 83 whole semen samples (86%) and 15 of 18 mature sperm swim-up samples (83%). However, high quality RNA was isolated from only 7 of 18 Percoll gradient sperm samples (39%). Interestingly quantitative reverse transcriptase-polymerase chain reaction analysis of 4 somatic and 10 germ cell markers showed that whole semen and swim-up samples had similar RNA profiles. RNA sequencing revealed that most encoded proteins were involved in mature sperm function, regulation of DNA replication, transcription, translation, cell cycle and embryo development.
conclusionsWe believe that semen and sperm specific RNAs are highly informative biomarkers for germ cell stages and somatic cell contribution. Therefore, these RNAs could be valuable diagnostic indicators of sperm survival, fertilization and early embryogenesis, and could serve as a predictor of the in vitro fertilization prognosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.