ArticleNature communications2014
Proton-coupled sugar transport in the prototypical major facilitator superfamily protein XylE.
Article in Nature communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.
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Who cites it
88 citing papers in PubMed.
- Substrate binding reorganizes the energetic landscape of Plasmodium falciparum hexose transporter PfHT1.bioRxiv : the preprint server for biology · 2026Article
- EngineeringSynthetic and systems biotechnology · 2026Article
- Proton-coupled alternating access in a versatile mycobacterial Spns drug transporter.Research square · 2026Article
- Cell-Surface and Periplasmic Deconstruction of Xylan by Oligotrophic Bacterium Caulobacter vibrioides.Environmental microbiology · 2026Article
- Proton-coupled alternating access in a versatile mycobacterial Spns drug transporter.bioRxiv : the preprint server for biology · 2026Article
- Characterization of three glycerol transporters with broad sugar specificities from Aspergillus niger.Applied microbiology and biotechnology · 2026Article
- A two-step mechanism for sugar translocation.Nature structural & molecular biology · 2026Article
- Docking-guided and phosphomimetic rational engineering of glucose-insensitive xylose transporters from Trichoderma reesei.Microbial cell factories · 2026Article
- Genetically encoded biosensor enabled mining, characterisation and engineering of aromatic acid MFS transporters.Journal of biological engineering · 2025Article
- Maltose and Maltotriose Transporters in Brewer'sInternational journal of molecular sciences · 2025Review
- Comparative analysis of STP6 and STP10 unravels molecular selectivity in sugar transport proteins.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Advances in fungal sugar transporters: unlocking the potential of second-generation bioethanol production.Applied microbiology and biotechnology · 2025Review
- Mutational analysis in Corynebacterium stationis MFS transporters for improving nucleotide bioproduction.Applied microbiology and biotechnology · 2024Article
- Structural and biochemical insights of xylose MFS and SWEET transporters in microbial cell factories: challenges to lignocellulosic hydrolysates fermentation.Frontiers in microbiology · 2024Review
- The role of salt bridge networks in the stability of the yeast hexose transporter 1.Biochimica et biophysica acta. General subjects · 2023Article
- Cysteine facilitates the lignocellulolytic response of Trichoderma guizhouense NJAU4742 by indirectly up-regulating membrane sugar transporters.Biotechnology for biofuels and bioproducts · 2023Article
- Structural basis for triacylglyceride extraction from mycobacterial inner membrane by MFS transporter Rv1410.Nature communications · 2023Article
- Establishing mammalian GLUT kinetics and lipid composition influences in a reconstituted-liposome system.Nature communications · 2023Article
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28 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
Abstract
The major facilitator superfamily (MFS) is the largest collection of structurally related membrane proteins that transport a wide array of substrates. The proton-coupled sugar transporter XylE is the first member of the MFS that has been structurally characterized in multiple transporting conformations, including both the outward and inward-facing states. Here we report the crystal structure of XylE in a new inward-facing open conformation, allowing us to visualize the rocker-switch movement of the N-domain against the C-domain during the transport cycle. Using molecular dynamics simulation, and functional transport assays, we describe the movement of XylE that facilitates sugar translocation across a lipid membrane and identify the likely candidate proton-coupling residues as the conserved Asp27 and Arg133. This study addresses the structural basis for proton-coupled substrate transport and release mechanism for the sugar porter family of proteins.
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