ArticleExperimental cell research2014
Direct observation of α-actinin tension and recruitment at focal adhesions during contact growth.
Article in Experimental cell research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 46 citations in OpenAlex.
- Acidosis Regulates Microtubule Dynamics via the β1 Integrin/RhoA/CRMP-2 Axis.Journal of the American Chemical Society · 2026Article
- Reconstituted nascent adhesion condensates drive actin polymerization on supported lipid bilayers.Science advances · 2026Article
- Cell shape and maturation impacts α-actinin-2 tension in iPSC-derived cardiomyocytes.APL bioengineering · 2026Article
- Structural and functional insights into α-actinin isoforms and their implications in cardiovascular disease.The Journal of general physiology · 2025Review
- Thermosensory Spiking Activity of Proteinoid Microspheres Cross-Linked by Actin Filaments.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Molecular Force Sensors for Biological Application.International journal of molecular sciences · 2024Review
- Visualizing Neurons Under Tension In Vivo with Optogenetic Molecular Force Sensors.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Epithelial cells sense local stiffness via Piezo1 mediated cytoskeletal reorganization.Frontiers in cell and developmental biology · 2023Article
- Merging of ventral fibers at adhesions drives the remodeling of cellular contractile systems in fibroblasts.Cytoskeleton (Hoboken, N.J.) · 2022Article
- Adherent cell remodeling on micropatterns is modulated by Piezo1 channels.Scientific reports · 2021Article
- Manipulation of Focal Adhesion Signaling by Pathogenic Microbes.International journal of molecular sciences · 2021Review
- Collagen, stiffness, and adhesion: the evolutionary basis of vertebrate mechanobiology.Molecular biology of the cell · 2020Review
- Different Vinculin Binding Sites Use the Same Mechanism to Regulate Directional Force Transduction.Biophysical journal · 2020Article
- Fluids and their mechanics in tumour transit: shaping metastasis.Nature reviews. Cancer · 2020Review
- When Stiffness Matters: Mechanosensing in Heart Development and Disease.Frontiers in cell and developmental biology · 2020Review
- A Review ofFrontiers in bioengineering and biotechnology · 2019Review
- Dissipation of contractile forces: the missing piece in cell mechanics.Molecular biology of the cell · 2017Article
- Mechanochemical Signaling Directs Cell-Shape Change.Biophysical journal · 2017Review
- Molecular mechanisms of mechanotransduction in integrin-mediated cell-matrix adhesion.Experimental cell research · 2016Review
- ROCK Inhibition Promotes Attachment, Proliferation, and Wound Closure in Human Embryonic Stem Cell-Derived Retinal Pigmented Epithelium.Translational vision science & technology · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Adherent cells interact with extracellular matrix via cell-substrate contacts at focal adhesions. The dynamic assembly and disassembly of focal adhesions enables cell attachment, migration and growth. While the influence of mechanical forces on the formation and growth of focal adhesions has been widely observed, the force loading on specific proteins at focal adhesion complex is not clear. By co-expressing force sensitive α-actinin FRET probes and fluorescence labeled paxillin in MDCK cells, we have simultaneously observed the time-dependent changes in tension in α-actinin and the dynamics of focal adhesion during cell migration. We show that increase in tension in α-actinin at the focal adhesion coincides with elongation of the adhesion in its growth phase. The enlargement of focal adhesion is through a force sensitive recruitment of α-actinin and paxillin to the adhesion sites. Changes in α-actinin tension and correlated relocation of α-actinin in an active adhesion also guide the growth direction of the adhesion. The results support the model that cytoskeletal tension is coupled to focal adhesion via the linking protein, α-actinin at the adhesion complex. Lysophosphatidic acid caused an immediate increase in α-actinin tension followed by drastic focal adhesion formation and elongation. Application of Rho-ROCK inhibitor, Y27632, resulted in reversible reduction in tension in α-actinin and disassociation of focal adhesion, suggesting the involvement of myosin-II mediated contractile force in the focal adhesion dynamics. These findings suggest that α-actinin not only serves as a physical linker between cytoskeleton and integrin, but also participates in force transmission at adhesion sites to facilitate adhesion׳s growth.
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Registered trials
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