ArticleScientific reports2014
Excited singlet molecular O₂(¹Δg) is generated enzymatically from excited carbonyls in the dark.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 64 citations in OpenAlex.
- Intracellular Singlet Oxygen Generation Mediated by Upconversion Nanoparticles in RAW 264.7 Macrophages.ACS omega · 2026Article
- Chemiexcitation in Ex Vivo Porcine Skin Model.Pigment cell & melanoma research · 2025Article
- Salicylic acid and ROS signaling modulate hypocotyl elongation in darkness via NPR1 and EX1.Science advances · 2025Article
- Analysis of Singlet Oxygen Luminescence Generated By Protoporphyrin IX.Antioxidants (Basel, Switzerland) · 2025Article
- Plasmalogen oxidation induces the generation of excited molecules and electrophilic lipid species.PNAS nexus · 2024Article
- The Chemical Reactivity of Membrane Lipids.Chemical reviews · 2024Review
- Astaxanthin: Past, Present, and Future.Marine drugs · 2023Review
- Colloidal Quantum Dots as an Emerging Vast Platform and Versatile Sensitizer for Singlet Molecular Oxygen Generation.ACS omega · 2023Review
- Chemiexcited Neurotransmitters and Hormones Create DNA Photoproducts in the Dark.ACS chemical biology · 2023Article
- Chemiexcitation: Mammalian Photochemistry in the DarkPhotochemistry and photobiology · 2023Review
- The application and trend of ultra-weak photon emission in biology and medicine.Frontiers in chemistry · 2023Review
- Radical pairs may play a role in microtubule reorganization.Scientific reports · 2022Article
- Regulation of Ion Channel Function by Gas Molecules.Advances in experimental medicine and biology · 2021Review
- Findings in redox biology: From HThe Journal of biological chemistry · 2020Article
- Reactive oxygen species (ROS) as pleiotropic physiological signalling agents.Nature reviews. Molecular cell biology · 2020Review
- Mechanism of the Formation of Electronically Excited Species by Oxidative Metabolic Processes: Role of Reactive Oxygen Species.Biomolecules · 2019Review
- Article
- Synthesis of meso-tetra-(4-sulfonatophenyl) porphyrin (TPPSInternational journal of nanomedicine · 2019Article
- Singlet oxygen modification abolishes voltage-dependent inactivation of the sea urchin spHCN channel.The Journal of general physiology · 2018Article
- Review of the methods for determination of reactive oxygen species and suggestion for their application in advanced oxidation induced by dielectric barrier discharges.Environmental science and pollution research international · 2018Article
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In mammalian tissues, ultraweak chemiluminescence arising from biomolecule oxidation has been attributed to the radiative deactivation of singlet molecular oxygen [O2 ((1)Δg)] and electronically excited triplet carbonyl products involving dioxetane intermediates. Herein, we describe evidence of the generation of O2 ((1)Δg) in aqueous solution via energy transfer from excited triplet acetone. This involves thermolysis of 3,3,4,4-tetramethyl-1,2-dioxetane, a chemical source, and horseradish peroxidase-catalyzed oxidation of 2-methylpropanal, as an enzymatic source. Both sources of excited carbonyls showed characteristic light emission at 1,270 nm, directly indicative of the monomolecular decay of O2 ((1)Δg). Indirect analysis of O2 ((1)Δg) by electron paramagnetic resonance using the chemical trap 2,2,6,6-tetramethylpiperidine showed the formation of 2,2,6,6-tetramethylpiperidine-1-oxyl. Using [(18)O]-labeled triplet, ground state molecular oxygen [(18)O2 ((3)Σg(-))], chemical trapping of (18)O2 ((1)Δg) with disodium salt of anthracene-9,10-diyldiethane-2,1-diyl disulfate yielding the corresponding double-[(18)O]-labeled 9,10-endoperoxide, was detected through mass spectrometry. This corroborates formation of O2 ((1)Δg). Altogether, photoemission and chemical trapping studies clearly demonstrate that chemically and enzymatically nascent excited carbonyl generates (18)O2 ((1)Δg) by triplet-triplet energy transfer to ground state oxygen O2 ((3)Σg(-)), and supports the long formulated hypothesis of O2 ((1)Δg) involvement in physiological and pathophysiological events that might take place in tissues in the absence of light.
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