Evidence map›Paper›PMID 25086780›Full record

ArticleThe Journal of nutritional biochemistry2014

Postnatal exposure to a high-carbohydrate diet interferes epigenetically with thyroid hormone receptor induction of the adult male rat skeletal muscle glucose transporter isoform 4 expression.

Nupur Raychaudhuri, Shanthie Thamotharan, Malathi Srinivasan, Saleh Mahmood, Mulchand S Patel, Sherin U Devaskar

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of nutritional biochemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it, 21 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Review
  5. A Type 2 Deiodinase-Dependent Increase inThyroid : official journal of the American Thyroid Association · 2021
    Article
  6. Review
  7. Review
  8. Sirtuins and Insulin Resistance.Frontiers in endocrinology · 2018
    Review
  9. Article
  10. Review
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Nupur RaychaudhuriDepartment of Pediatrics, Division of Neonatology & Developmental Biology, Neonatal Research Center, David Geffen School of Medicine UCLA, Los Angeles, CA 90095-1752, USA.
Shanthie ThamotharanDepartment of Pediatrics, Division of Neonatology & Developmental Biology, Neonatal Research Center, David Geffen School of Medicine UCLA, Los Angeles, CA 90095-1752, USA.
Malathi SrinivasanDepartment of Biochemistry, School of Medicine and Biomedical Sciences, University at Buffalo, State University of New York, Buffalo, NY 14214, USA.
Saleh MahmoodDepartment of Biochemistry, School of Medicine and Biomedical Sciences, University at Buffalo, State University of New York, Buffalo, NY 14214, USA.
Mulchand S PatelDepartment of Biochemistry, School of Medicine and Biomedical Sciences, University at Buffalo, State University of New York, Buffalo, NY 14214, USA.
Sherin U DevaskarDepartment of Pediatrics, Division of Neonatology & Developmental Biology, Neonatal Research Center, David Geffen School of Medicine UCLA, Los Angeles, CA 90095-1752, USA. Electronic address: sdevaskar@mednet.ucla.edu.
University at Buffalo, State University of New York · USUniversity of California, Los Angeles · US

Funding

Prenatal Metabolic Programming of the OffspringR01HD041230 · NICHD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DEVASKAR, SHERIN U · 2001 to 2019
$5.2M
Maternal Hyperinsulinemia and Fetal ProgrammingR01DK061518 · NIDDK · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI PATEL, MULCHAND S · 2001 to 2011
$3.1M
PERINATAL METABOLIC AND HORMONAL EFFECTSR01HD025024 · NICHD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DEVASKAR, SHERIN U · 1996 to 2010
$2.6M
DEVELOPMENTAL REGULATION OF NEURON INSULIN-LIKE PEPTIDER29HD025024 · NICHD · SAINT LOUIS UNIVERSITY · PI DEVASKAR, SHERIN U. · 1989 to 1993
–
NICHD NIH HHS HD25024NICHD NIH HHS HD41230NICHD NIH HHS R01 HD025024NICHD NIH HHS R01 HD041230NIDDK NIH HHS DK61518NIDDK NIH HHS R01 DK061518
6 · The paper itself

Abstract

Early life nutritional intervention causes adult-onset insulin resistance and obesity in rats. Thyroid hormone receptor (TR), in turn, transcriptionally enhances skeletal muscle Glut4 expression. We tested the hypothesis that reduced circulating thyroid-stimulating hormone and T4 concentrations encountered in postnatal (PN4-PN24) high-carbohydrate (HC) milk formula-fed versus the mother-fed controls (MF) would epigenetically interfere with TR induction of adult (100 days) male rat skeletal muscle Glut4 expression, thereby providing a molecular mechanism mediating insulin resistance. We observed increased DNA methylation of the CpG island with enhanced recruitment of Dnmt3a, Dnmt3b and MeCP2 in the glut4 promoter region along with reduced acetylation of histone (H)2A.Z and H4 particularly at the H4.lysine (K)16 residue, which was predominantly mediated by histone deacetylase 4 (HDAC4). This was followed by enhanced recruitment of heterochromatin protein 1β to the glut4 promoter with increased Suv39H1 methylase concentrations. These changes reduced TR binding of the T3 response element of the glut4 gene (TREs; -473 to -450 bp) detected qualitatively in vivo (electromobility shift assay) and quantified ex vivo (chromatin immunoprecipitation). In addition, the recruitment of steroid receptor coactivator and CREB-binding protein to the glut4 promoter-protein complex was reduced. Co-immunoprecipitation experiments confirmed the interaction between TR and CBP to be reduced and HDAC4 to be enhanced in HC versus MF groups. These molecular changes were associated with diminished skeletal muscle Glut4 mRNA and protein concentrations. We conclude that early postnatal exposure to HC diet epigenetically reduced TR induction of adult male skeletal muscle Glut4 expression, uncovering novel molecular mechanisms contributing to adult insulin resistance and obesity.

Indexed as

Epigenesis, GeneticAnimalsAnimals, NewbornCells, CulturedCpG IslandsCREB-Binding ProteinDietary CarbohydratesDNA MethylationGlucose Transporter Type 4Histone DeacetylasesHistonesImmunoprecipitationInsulin ResistanceMaleMuscle, SkeletalPostnatal CareCREB-Binding ProteinCrebbp protein, ratDietary CarbohydratesGlucose Transporter Type 4HDAC4 protein, ratHistone DeacetylasesHistonesReceptors, Thyroid HormoneRNA, MessengerSlc2a4 protein, ratThyroxineTriiodothyronineDNA methylaseDNA methylationHigh Carbohydrate programmingHistone code modificationsHistone deacetylaseInsulin resistanceObesity

Identifiers

PMID25086780
PMCPMC4537062
OpenAlexW1999058632

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.