ArticleCurrent microbiology2014
Sequence analysis and characterization of plasmid pLK39 isolated from endophytic Salmonella sp. isolated from Solanum lycocarpum.
Article in Current microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed, 1 citations in OpenAlex.
- Bacteriological water quality in school's drinking fountains and detection antibiotic resistance genes.Annals of clinical microbiology and antimicrobials · 2017Article
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Authors and funding
4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The complete nucleotide sequence of a small cryptic plasmid pLK39 isolated from endophytic Salmonella sp. was determined. This plasmid is 4,029 bp long with an overall GC content of 55.4 %. Sequence analyses of pLK39 revealed extensive homology to several plasmids: pRK10, pK, pSW200, pBERT, pST728/06-2, pSW100, pEC3, and pUCD5000. Using the ORF Finder program, 35 putative ORFs was identified, 30 showed more than 35 residues. After performing a search for homologous sequences to the pLK39 at BLASTn software on NCBI, it was ascertained that the plasmid has a ColE1-like replication origin and also a region of mobilization proteins from relaxase family (mobCABD). Besides these mobilization proteins, the pLK39 codes a putative DUF903 protein family, which is characterized as assumed external cytoplasmic membrane lipoprotein. A recombinant form of pLK39 carrying a kanamycin resistance gene is stably maintained in Escherichia coli cells grown in the absence of selection pressure. pLK39 was compatible with pUC18, pBR322, and pACYC184.
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