ArticleCurrent microbiology2014
Development of double loop-mediated isothermal amplification to detect Listeria monocytogenes in food.
Article in Current microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Development and validation of a rapidFrontiers in microbiology · 2026Article
- Listeria monocytogenes an Emerging Pathogen: a Comprehensive Overview on Listeriosis, Virulence Determinants, Detection, and Anti-Listerial Interventions.Microbial ecology · 2023Review
- Application of a Loop-Mediated Isothermal Amplification (LAMP) Assay for the Detection ofFoods (Basel, Switzerland) · 2023Article
- Nano-gold-enhanced LAMP method for qualitative visual detection of Salmonella in milk.Mikrochimica acta · 2022Article
- Review
- Rapid and Specific Detection of the Poplar Black Spot Disease Caused byPlants (Basel, Switzerland) · 2021Article
- Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection ofFrontiers in microbiology · 2021Article
- Loop-Mediated Isothermal Amplification (LAMP) for the Diagnosis of Zika Virus: A Review.Viruses · 2019Review
- Loop-mediated isothermal amplification (LAMP): a versatile technique for detection of micro-organisms.Journal of applied microbiology · 2018Review
- Identification of Methicillin-Resistant Staphylococcus aureus (MRSA) Using Simultaneous Detection of mecA, nuc, and femB by Loop-Mediated Isothermal Amplification (LAMP).Current microbiology · 2017Article
- Evaluation of Two Loop-mediated Isothermal Amplification Methods for the Detection ofItalian journal of food safety · 2015Article
- An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food.Frontiers in microbiology · 2015Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, a double loop-mediated isothermal amplification (dLAMP) based on two target genes hlyA and iap was developed for the rapid detection of Listeria monocytogenes in food. The results revealed that the detection time and temperature of our dLAMP assay for L. monocytogenes were 15 min and 63 °C respectively, with a sensitivity of 10 fg DNA of L. monocytogenes per tube. While normal LAMP (nLAMP) of hlyA or iap was 100 fg DNA of L. monocytogenes per tube for 45 min and 63 °C. Furthermore, mineral oil and GoldViewII nucleic acid stain were chosen as the basic materials to develop a simple visualized identification of the positive samples. A total of 450 food samples were tested for L. monocytogenes using the dLAMP protocol developed in this study. The results showed that the accuracy of the dLAMP and the "gold standard" culture-biotechnical method were 100 % identical, suggesting that the modified dLAMP assay would provide a potential for detection of L. monocytogenes in food products.
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