Evidence map›Paper›PMID 25086504›Full record

ArticleNature methods2014

Nanoscale high-content analysis using compositional heterogeneities of single proteoliposomes.

Signe Mathiasen, Sune M Christensen, Juan José Fung, Søren G F Rasmussen, Jonathan F Fay, Sune K Jorgensen, Salome Veshaguri, David L Farrens, Maria Kiskowski, Brian Kobilka and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.

0numbers the graph read from it
0cells of the map it votes in
37citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

37 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Application of Single Extracellular Vesicle Analysis Techniques.International journal of nanomedicine · 2023
    Review
  10. Article
  11. Article
  12. Review
  13. Interrogating surfaceChemical science · 2020
    Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Signe Mathiasen1] Department of Chemistry, University of Copenhagen, Copenhagen, Denmark. [2] Nano-Science Center, University of Copenhagen, Copenhagen, Denmark. [3] Lundbeck Foundation Center for Biomembranes in Nanomedicine, University of Copenhagen, Copenhagen, Denmark.
Sune M Christensen1] Department of Chemistry, University of Copenhagen, Copenhagen, Denmark. [2] Nano-Science Center, University of Copenhagen, Copenhagen, Denmark. [3] Lundbeck Foundation Center for Biomembranes in Nanomedicine, University of Copenhagen, Copenhagen, Denmark.
Juan José Fung1] Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Palo Alto, California, USA. [2].
Søren G F Rasmussen1] Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Palo Alto, California, USA. [2] Department of Neuroscience and Pharmacology, University of Copenhagen, Copenhagen, Denmark.
Jonathan F FayDepartment of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, Oregon, USA.
Sune K Jorgensen1] Department of Chemistry, University of Copenhagen, Copenhagen, Denmark. [2] Nano-Science Center, University of Copenhagen, Copenhagen, Denmark. [3] Lundbeck Foundation Center for Biomembranes in Nanomedicine, University of Copenhagen, Copenhagen, Denmark.
Salome Veshaguri1] Department of Chemistry, University of Copenhagen, Copenhagen, Denmark. [2] Nano-Science Center, University of Copenhagen, Copenhagen, Denmark. [3] Lundbeck Foundation Center for Biomembranes in Nanomedicine, University of Copenhagen, Copenhagen, Denmark.
David L FarrensDepartment of Biochemistry and Molecular Biology, Oregon Health and Science University, Portland, Oregon, USA.
Maria KiskowskiDepartment of Mathematics and Statistics, University of South Alabama, Mobile, Alabama, USA.
Brian KobilkaDepartment of Molecular and Cellular Physiology, Stanford University School of Medicine, Palo Alto, California, USA.
Dimitrios Stamou1] Department of Chemistry, University of Copenhagen, Copenhagen, Denmark. [2] Nano-Science Center, University of Copenhagen, Copenhagen, Denmark. [3] Lundbeck Foundation Center for Biomembranes in Nanomedicine, University of Copenhagen, Copenhagen, Denmark.

Funding

BIOLOGICAL BASES OF DRUG-SEEKING BEHAVIORT32DA007262 · NIDA · OREGON HEALTH & SCIENCE UNIVERSITY · PI WILLIAMS, JOHN T · 1991 to 2022
$9.4M
THE MECHANISM OF BETA2 ADRENOCEPTOR ACTIVATIONR01NS028471 · NINDS · STANFORD UNIVERSITY · PI KOBILKA, BRIAN K · 1990 to 2021
$6.7M
NIDA NIH HHS T32 DA007262NINDS NIH HHS R01 NS028471
6 · The paper itself

Abstract

Proteoliposome reconstitution is a standard method to stabilize purified transmembrane proteins in membranes for structural and functional assays. Here we quantified intrareconstitution heterogeneities in single proteoliposomes using fluorescence microscopy. Our results suggest that compositional heterogeneities can severely skew ensemble-average proteoliposome measurements but also enable ultraminiaturized high-content screens. We took advantage of this screening capability to map the oligomerization energy of the β2-adrenergic receptor using ∼10(9)-fold less protein than conventional assays.

Indexed as

AlgorithmsImage Interpretation, Computer-AssistedMicroscopy, FluorescenceNanotechnologyProteolipidsReceptors, G-Protein-CoupledSpectrometry, FluorescenceProteolipidsproteoliposomesReceptors, G-Protein-Coupled

Identifiers

PMID25086504
PMCPMC4485457

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.