ArticleBiomaterials2014
The combined effects of matrix stiffness and growth factor immobilization on the bioactivity and differentiation capabilities of adipose-derived stem cells.
Article in Biomaterials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 78 citations in OpenAlex.
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- Local photocrosslinking of native tissue matrix regulates lung epithelial cell mechanosensing and function.Nature materials · 2025Article
- Human bone marrow derived stem cell differentiation on 3D printed bioactive glass scaffolds.Journal of materials science. Materials in medicine · 2025Article
- Advancements in hydrogel design for articular cartilage regeneration: A comprehensive review.Bioactive materials · 2025Review
- The Role of Bioceramics for Bone Regeneration: History, Mechanisms, and Future Perspectives.Biomimetics (Basel, Switzerland) · 2024Review
- Nanofiber matrix formulations for the delivery of Exendin-4 for tendon regeneration:Bioactive materials · 2023Article
- Photocrosslinkable natural polymers in tissue engineering.Frontiers in bioengineering and biotechnology · 2023Review
- Effect of Polymeric Matrix Stiffness on Osteogenic Differentiation of Mesenchymal Stem/Progenitor Cells: Concise Review.Polymers · 2021Review
- Advances of adipose-derived mesenchymal stem cells-based biomaterial scaffolds for oral and maxillofacial tissue engineering.Bioactive materials · 2021Review
- Alteration of 3D Matrix Stiffness Regulates Viscoelasticity of Human Mesenchymal Stem Cells.International journal of molecular sciences · 2021Article
- Inclusion of a 3D-printed Hyperelastic Bone mesh improves mechanical and osteogenic performance of a mineralized collagen scaffold.Acta biomaterialia · 2021Article
- Immobilization of Growth Factors for Cell Therapy Manufacturing.Frontiers in bioengineering and biotechnology · 2020Review
- Sequential sequestrations increase the incorporation and retention of multiple growth factors in mineralized collagen scaffolds.RSC advances · 2020Article
- Current Strategies to Enhance Adipose Stem Cell Function: An Update.International journal of molecular sciences · 2019Review
- Preparation of an acellular spinal cord scaffold to improve its biological properties.Molecular medicine reports · 2019Article
- The inclusion of zinc into mineralized collagen scaffolds for craniofacial bone repair applications.Acta biomaterialia · 2019Article
- Progress in three-dimensional printing with growth factors.Journal of controlled release : official journal of the Controlled Release Society · 2019Review
- Leveraging Biomaterial Mechanics to Improve Pluripotent Stem Cell Applications for Tissue Engineering.Frontiers in bioengineering and biotechnology · 2019Article
- Phage-Based Artificial Niche: The Recent Progress and Future Opportunities in Stem Cell Therapy.Stem cells international · 2019Review
- Incorporating β-cyclodextrin into collagen scaffolds to sequester growth factors and modulate mesenchymal stem cell activity.Acta biomaterialia · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Biomaterial designs are increasingly incorporating multiple instructive signals to induce a desired cell response. However, many approaches do not allow orthogonal manipulation of immobilized growth factor signals and matrix stiffness. Further, few methods support patterning of biomolecular signals across a biomaterial in a spatially-selective manner. Here, we report a sequential approach employing carbodiimide crosslinking and benzophenone photoimmobilization chemistries to orthogonally modify the stiffness and immobilized growth factor content of a model collagen-GAG (CG) biomaterial. We subsequently examined the singular and combined effects of bone morphogenetic protein (BMP-2), platelet derived growth factor (PDGF-BB), and CG membrane stiffness on the bioactivity and osteogenic/adipogenic lineage-specific gene expression of adipose derived stem cells, an increasingly popular cell source for regenerative medicine studies. We found that the stiffest substrates direct osteogenic lineage commitment of ASCs regardless of the presence or absence of growth factors, while softer substrates require biochemical cues to direct cell fate. We subsequently describe the use of this approach to create overlapping patterns of growth factors across a single substrate. These results highlight the need for versatile approaches to selectively manipulate the biomaterial microenvironment to identify synergies between biochemical and mechanical cues for a range of regenerative medicine applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.