Evidence map›Paper›PMID 25085858›Full record

ArticleBiomaterials2014

Engineering vascular tissue with functional smooth muscle cells derived from human iPS cells and nanofibrous scaffolds.

Yongyu Wang, Jiang Hu, Jiao Jiao, Zhongning Liu, Zhou Zhou, Chao Zhao, Lung-Ji Chang, Y Eugene Chen, Peter X Ma, Bo Yang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.

0numbers the graph read from it
0cells of the map it votes in
60citing papers in PubMed
7.9field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

60 citing papers in PubMed, 129 citations in OpenAlex.

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  18. 2D Materials for Cardiac Tissue Repair and Regeneration.Frontiers in cardiovascular medicine · 2022
    Review
  19. Methods for Differentiating hiPSCs into Vascular Smooth Muscle Cells.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Yongyu WangDepartment of Cardiac Surgery, Cardiovascular Center, The University of Michigan, Ann Arbor, MI 48109, USA.
Jiang HuDepartment of Biologic and Materials Sciences, The University of Michigan, Ann Arbor, MI 48109, USA.
Jiao JiaoDepartment of Cardiac Surgery, Cardiovascular Center, The University of Michigan, Ann Arbor, MI 48109, USA.
Zhongning LiuDepartment of Biologic and Materials Sciences, The University of Michigan, Ann Arbor, MI 48109, USA.
Zhou ZhouDepartment of Cardiac Surgery, Cardiovascular Center, The University of Michigan, Ann Arbor, MI 48109, USA.
Chao ZhaoDepartment of Biologic and Materials Sciences, The University of Michigan, Ann Arbor, MI 48109, USA.
Lung-Ji ChangDepartment of Molecular Genetics & Microbiology, University of Florida, Gainesville, FL 32610, USA.
Y Eugene ChenDepartment of Cardiac Surgery, Cardiovascular Center, The University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: echenum@umich.edu.
Peter X MaDepartment of Biologic and Materials Sciences, The University of Michigan, Ann Arbor, MI 48109, USA; Department of Biomedical Engineering, The University of Michigan, Ann Arbor, MI 48109, USA; Macromolecular Science and Engineering Center, The University of Michigan, Ann Arbor, MI 48109, USA; Department of Materials Science and Engineering, The University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: mapx@umich.edu.
Bo YangDepartment of Cardiac Surgery, Cardiovascular Center, The University of Michigan, Ann Arbor, MI 48109, USA. Electronic address: boya@umich.edu.
University of Michigan–Ann Arbor · USUniversity of Florida · US

Funding

Regenerating Blood Vessels Using iPS CellsR01HL114038 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI MA, PETER X · 2013 to 2016
$2.1M
NHLBI NIH HHS HL114038NHLBI NIH HHS R01 HL114038
6 · The paper itself

Abstract

Tissue-engineered blood vessels (TEBVs) are promising in the replacement of diseased vascular tissues. However, it remains a great challenge to obtain a sufficient number of functional smooth muscle cells (SMCs) in a clinical setting to construct patient-specific TEBVs. In addition, it is critical to develop a scaffold to accommodate these cells and retain their functional phenotype for the regeneration of TEBVs. In this study, human induced pluripotent stem cells (iPSCs) were established from primary human aortic fibroblasts, and characterized with the pluripotency markers expression and cells' capabilities to differentiate into all three germ layer cells. A highly efficient method was then developed to induce these human iPSCs into proliferative SMCs. After multiple times of expansion, the expanded SMCs retained the potential to be induced into the functional contractile phenotype of mature SMCs, which was characterized by the contractile response to carbachol treatment, up-regulation of specific collagen genes under transforming growth factor β1 treatment, and up-regulation of specific matrix metalloproteinase genes under cytokine stimulation. We also developed an advanced macroporous and nanofibrous (NF) poly(l-lactic acid) (PLLA) scaffold with suitable pore size and interpore connectivity to seed these human iPSC-derived SMCs and maintain their differentiated phenotype. Subcutaneous implantation of the SMC-scaffold construct in nude mice demonstrated vascular tissue formation, with robust collagenous matrix deposition inside the scaffold and the maintenance of differentiated SMC phenotype. Taken together, this study established an exciting approach towards the construction of patient-specific TEBVs. We established patient-specific human iPSCs, derived proliferative SMCs for expansion, turned on their mature contractile SMC phenotype, and developed an advanced scaffold for these cells to regenerate vascular tissue in vivo.

Indexed as

Blood VesselsCell DifferentiationCell ProliferationCells, CulturedFibroblastsHumansInduced Pluripotent Stem CellsLactic AcidMicroscopy, Electron, ScanningMuscle ContractionMyocytes, Smooth MuscleNanofibersPhenotypePolyestersPolymersRegenerationLactic AcidPolyesterspoly(lactide)PolymersTransforming Growth Factor beta1Human induced pluripotent stem cellNanofibrous scaffoldSmooth muscle cellTissue-engineered vascular tissue

Identifiers

PMID25085858
PMCPMC4140627
OpenAlexW2154791520

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.