ArticleJournal of microbiology (Seoul, Korea)2014
Pseudomonas aeruginosa MdaB and WrbA are water-soluble two-electron quinone oxidoreductases with the potential to defend against oxidative stress.
Article in Journal of microbiology (Seoul, Korea), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- The two-component system ColRS discriminates metal signals to regulate virulence and resistance in Pseudomonas aeruginosa.PLoS pathogens · 2026Article
- Purifying selection and low recombination facilitated sequential colonization of benthic and pelagic coastal ocean by ammonia-oxidizing archaea.ISME communications · 2025Article
- Cyclic Isothiocyanate Goitrin ImpairsPlants (Basel, Switzerland) · 2024Article
- The Azotobacter vinelandii AlgU regulon during vegetative growth and encysting conditions: A proteomic approach.PloS one · 2023Article
- Effects of the Quinone Oxidoreductase WrbA onAntioxidants (Basel, Switzerland) · 2021Article
- Plasma Membrane MCC/Eisosome Domains Promote Stress Resistance in Fungi.Microbiology and molecular biology reviews : MMBR · 2020Review
- Diazaquinomycin Biosynthetic Gene Clusters from Marine and Freshwater Actinomycetes.Journal of natural products · 2019Article
- NAD(P)H quinone oxidoreductase (NQO1): an enzyme which needs just enough mobility, in just the right places.Bioscience reports · 2019Review
- Quantitative Proteomics of the 2016 WHOMolecular & cellular proteomics : MCP · 2019Article
- Pseudomonas aeruginosa ttcA encoding tRNA-thiolating protein requires an iron-sulfur cluster to participate in hydrogen peroxide-mediated stress protection and pathogenicity.Scientific reports · 2018Article
- Identification of a Small Molecule Anti-biofilm Agent AgainstFrontiers in microbiology · 2018Article
- Functional Annotation of a Presumed Nitronate Monoxygenase Reveals a New Class of NADH:Quinone Reductases.The Journal of biological chemistry · 2016Article
- WrpA Is an Atypical Flavodoxin Family Protein under Regulatory Control of the Brucella abortus General Stress Response System.Journal of bacteriology · 2016Article
- Flavodoxin-Like Proteins Protect Candida albicans from Oxidative Stress and Promote Virulence.PLoS pathogens · 2015Article
- A novel cytosolic NADH:quinone oxidoreductase from Methanothermobacter marburgensis.Bioscience reports · 2014Article
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Water-soluble quinone oxidoreductases capable of reducing quinone substrates via a concerted two-electron mechanism have been implicated in bacterial antioxidant defence. Twoelectron transfer avoids formation of dangerously reactive semi-quinone intermediates, moreover previous work in Pseudomonas putida indicated a direct protective effect for the quinols generated by an over-expressed oxidoreductase. Here, the Pseudomonas aeruginosa orthologs of five quinone oxidoreductases--MdaB, ChrR, WrbA, NfsB, and NQO1--were tested for their possible role in defending P. aeruginosa against H2O2 challenge. In in vitro assays, each enzyme was shown to reduce quinone substrates with only minimal semiquinone formation. However, when each was individually over-expressed in P. aeruginosa no overt H2O2-protective phenotype was observed. It was shown that this was due to a masking effect of the P. aeruginosa catalase, KatA; in a katA mutant, H2O2 challenged strains over-expressing the WrbA and MdaB orthologs grew significantly better than the empty plasmid control. A growth advantage was also observed for H2O2 challenged P. putida strains over-expressing P. aeruginosa wrbA, mdaB or katA. Despite not conferring a growth advantage to wild type P. aeruginosa, it is possible that these quinone oxidoreductases defend against H2O2 toxicity at lower concentrations.
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