ArticleBMC microbiology2014
Characterization and analysis of the Burkholderia pseudomallei BsaN virulence regulon.
Article in BMC microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Regulation of Type VI Secretion Systems of Burkholderia during transition to intracellular lifestyle.EMBO reports · 2026Article
- Functional diversity of accessory factors of the type VI secretion system.Journal of bacteriology · 2026Review
- Secretion systems shaping host-pathogen interactions in Burkholderia species.Applied microbiology and biotechnology · 2026Review
- Article
- Virulome and phylogenomic profiling of a novel Burkholderia pseudomallei strain from an Indian clinical isolate.Molecular genetics and genomics : MGG · 2024Article
- Article
- The AraC/XylS Protein MxiE and Its Coregulator IpgC Control a Negative Feedback Loop in the Transcriptional Cascade That Regulates Type III Secretion in Shigella flexneri.Journal of bacteriology · 2022Article
- Identification of a PadR-type regulator essential for intracellular pathogenesis of Burkholderia pseudomallei.Scientific reports · 2021Article
- Genomic and RT-qPCR analysis of trimethoprim-sulfamethoxazole and meropenem resistance in Burkholderia pseudomallei clinical isolates.PLoS neglected tropical diseases · 2021Article
- Human Melioidosis.Clinical microbiology reviews · 2020Review
- An Evolutionary Arms Race BetweenFrontiers in microbiology · 2020Review
- An in situ high-throughput screen identifies inhibitors of intracellularProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Burkholderia thailandensis strain E555 is a surrogate for the investigation of Burkholderia pseudomallei replication and survival in macrophages.BMC microbiology · 2019Article
- GvmR - A Novel LysR-Type Transcriptional Regulator Involved in Virulence and Primary and Secondary Metabolism ofFrontiers in microbiology · 2018Article
- TheFrontiers in microbiology · 2018Review
- Review
- Global Awakening of Cryptic Biosynthetic Gene Clusters in Burkholderia thailandensis.ACS chemical biology · 2017Article
- Global and regional dissemination and evolution of Burkholderia pseudomallei.Nature microbiology · 2017Article
- Type III Secretion in the Melioidosis PathogenFrontiers in cellular and infection microbiology · 2017Review
- DBSecSys 2.0: a database of Burkholderia mallei and Burkholderia pseudomallei secretion systems.BMC bioinformatics · 2016Article
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Authors and funding
9 authors.
Funding
Abstract
backgroundBurkholderia pseudomallei is a facultative intracellular pathogen and the causative agent of melioidosis. A conserved type III secretion system (T3SS3) and type VI secretion system (T6SS1) are critical for intracellular survival and growth. The T3SS3 and T6SS1 genes are coordinately and hierarchically regulated by a TetR-type regulator, BspR. A central transcriptional regulator of the BspR regulatory cascade, BsaN, activates a subset of T3SS3 and T6SS1 loci.
resultsTo elucidate the scope of the BsaN regulon, we used RNAseq analysis to compare the transcriptomes of wild-type B. pseudomallei KHW and a bsaN deletion mutant. The 60 genes positively-regulated by BsaN include those that we had previously identified in addition to a polyketide biosynthesis locus and genes involved in amino acid biosynthesis. BsaN was also found to repress the transcription of 51 genes including flagellar motility loci and those encoding components of the T3SS3 apparatus. Using a promoter-lacZ fusion assay in E. coli, we show that BsaN together with the chaperone BicA directly control the expression of the T3SS3 translocon, effector and associated regulatory genes that are organized into at least five operons (BPSS1516-BPSS1552). Using a mutagenesis approach, a consensus regulatory motif in the promoter regions of BsaN-regulated genes was shown to be essential for transcriptional activation.
conclusionsBsaN/BicA functions as a central regulator of key virulence clusters in B. pseudomallei within a more extensive network of genetic regulation. We propose that BsaN/BicA controls a gene expression program that facilitates the adaption and intracellular survival of the pathogen within eukaryotic hosts.
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