ArticleApplied and environmental microbiology2014
Punicalagin inhibits Salmonella virulence factors and has anti-quorum-sensing potential.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 108 citations in OpenAlex.
- The Versatility of Pomegranate: From Phytochemical Diversity to Antimicrobial and Translational Applications.Microorganisms · 2026Review
- Berberine Hydrochloride Reduces the Intracellular Survival ofBiomolecules · 2025Article
- Antibiotic Alternatives and Next-Generation Therapeutics forAntibiotics (Basel, Switzerland) · 2025Review
- Identification of Punicalagin as a Key Bioactive Compound Responsible for the Antimicrobial Properties ofJournal of agricultural and food chemistry · 2025Article
- Multidrug Resistance and Virulence Traits ofAntibiotics (Basel, Switzerland) · 2025Article
- The eubiotic perspective on utilization of tannins in phytotherapy and nutrition of pigs.Frontiers in pharmacology · 2025Review
- Punicalagin, a pomegranate polyphenol sensitizes the activity of antibiotics against three MDR pathogens of the Enterobacteriaceae.BMC complementary medicine and therapies · 2024Article
- Salmonellosis: An Overview of Epidemiology, Pathogenesis, and Innovative Approaches to Mitigate the Antimicrobial Resistant Infections.Antibiotics (Basel, Switzerland) · 2024Review
- The Anti-Virulence Activities of the Antihypertensive Drug Propranolol in Light of Its Anti-Quorum Sensing Effects againstBiomedicines · 2023Article
- Review
- Antimicrobial Resistance and Recent Alternatives to Antibiotics for the Control of Bacterial Pathogens with an Emphasis on Foodborne Pathogens.Antibiotics (Basel, Switzerland) · 2023Review
- Antibiofilm effects of punicalagin againstFrontiers in microbiology · 2023Article
- Disalicylic Acid Provides Effective Control ofMicroorganisms · 2022Article
- Regulatory Mechanisms between Quorum Sensing and Virulence inMicroorganisms · 2022Review
- Propionate and Butyrate Inhibit Biofilm Formation ofFoods (Basel, Switzerland) · 2022Article
- RETRACTED: Characterization of the Anti-Biofilm and Anti-Quorum Sensing Activities of the β-Adrenoreceptor Antagonist Atenolol against Gram-Negative Bacterial Pathogens.International journal of molecular sciences · 2022Article
- The Role ofFoods (Basel, Switzerland) · 2022Article
- Punicalagin, an Inhibitor of Sortase A, Is a Promising Therapeutic Drug to Combat Methicillin-Resistant Staphylococcus aureus Infections.Antimicrobial agents and chemotherapy · 2022Article
- Phytochemical composition and antimicrobial, and anti-quorum sensing activities ofIranian journal of microbiology · 2022Article
- Mechanisms of Inhibition of Quorum Sensing as an Alternative for the Control ofMicroorganisms · 2022Review
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Punicalagin, an essential component of pomegranate rind, has been demonstrated to possess antimicrobial activity against several food-borne pathogens, but its activity on the virulence of pathogens and its anti-quorum-sensing (anti-QS) potential have been rarely reported. This study investigated the efficacy of subinhibitory concentrations of punicalagin on Salmonella virulence factors and QS systems. A broth microdilution method was used to determine the MICs of punicalagin for 10 Salmonella strains. Motility assay and quantitative reverse transcription (RT)-PCR were performed to evaluate the effects of punicalagin on the virulence attributes and QS-related genes of Salmonella. The MICs of punicalagin for several Salmonella strains ranged from 250 to 1,000 μg/ml. Motility assays showed that punicalagin, at 1/16× MIC and 1/32× MIC, significantly decreased bacterial swimming and swarming motility, which corresponded to downregulation of the motility-related genes (fliA, fliY, fljB, flhC, and fimD) in RT-PCR assays. RT-PCR also revealed that punicalagin downregulated the expression of most of the selected genes involved in Salmonella virulence. Moreover, a QS inhibition assay indicated that punicalagin dose dependently inhibited the production of violacein by Chromobacterium violaceum and repressed the expression of QS-related genes (sdiA and srgE) in Salmonella. In addition, punicalagin significantly reduced Salmonella invasion of colonic cells (P<0.01) with no impact on adhesion. These findings suggest that punicalagin has the potential to be developed as an alternative or supplemental agent for prevention of Salmonella infection.
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