ArticleApplied and environmental microbiology2014
Improvement of 2,3-butanediol yield in Klebsiella pneumoniae by deletion of the pyruvate formate-lyase gene.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 60 citations in OpenAlex.
- Systemic metabolic engineering of Enterobacter aerogenes for efficient 2,3-butanediol production.Applied microbiology and biotechnology · 2024Article
- Polymers without Petrochemicals: Sustainable Routes to Conventional Monomers.Chemical reviews · 2023Review
- Metabolic Engineering of Microorganisms to Produce Pyruvate and Derived Compounds.Molecules (Basel, Switzerland) · 2023Review
- Establishing aMolecules (Basel, Switzerland) · 2022Article
- Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in L-valine production.World journal of microbiology & biotechnology · 2022Article
- Improved production of 2,3-butanediol and isobutanol by engineering electron transport chain in Escherichia coli.Microbial biotechnology · 2021Article
- Metabolic Engineering ofFrontiers in microbiology · 2021Article
- Analysis of the Growth and Metabolites of a Pyruvate Dehydrogenase Complex- DeficientJournal of microbiology and biotechnology · 2020Article
- Regulation of Pyruvate Formate Lyase-Deficient Klebsiella pneumoniae for Efficient 1,3-Propanediol Bioproduction.Current microbiology · 2020Article
- Knockout of acetoacetate degradation pathway gene3 Biotech · 2019Article
- High-yield production of 1,3-propanediol from glycerol by metabolically engineeredBiotechnology for biofuels · 2018Article
- Holistic bioengineering: rewiring central metabolism for enhanced bioproduction.The Biochemical journal · 2017Review
- Metabolic engineering of Klebsiella pneumoniae based on in silico analysis and its pilot-scale application for 1,3-propanediol and 2,3-butanediol co-production.Journal of industrial microbiology & biotechnology · 2017Article
- Production of C2-C4 diols from renewable bioresources: new metabolic pathways and metabolic engineering strategies.Biotechnology for biofuels · 2017Review
- EngineeringBiotechnology for biofuels · 2017Article
- A Combinatorial Algorithm for Microbial Consortia Synthetic Design.Scientific reports · 2016Article
- Metabolic engineering of Escherichia coli for production of (2S,3S)-butane-2,3-diol from glucose.Biotechnology for biofuels · 2015Article
- Metabolic Engineering of Klebsiella pneumoniae for the Production of 2-Butanone from Glucose.PloS one · 2015Article
- Alleviation of carbon catabolite repression in Enterobacter aerogenes for efficient utilization of sugarcane molasses for 2,3-butanediol production.Biotechnology for biofuels · 2015Article
- High alcohol-producingGut microbesArticle
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Klebsiella pneumoniae is considered a good host strain for the production of 2,3-butanediol, which is a promising platform chemical with various industrial applications. In this study, three genes, including those encoding glucosyltransferase (wabG), lactate dehydrogenase (ldhA), and pyruvate formate-lyase (pflB), were disrupted in K. pneumoniae to reduce both its pathogenic characteristics and the production of several by-products. In flask cultivation with minimal medium, the yield of 2,3-butanediol from rationally engineered K. pneumoniae (ΔwabG ΔldhA ΔpflB) reached 0.461 g/g glucose, which was 92.2% of the theoretical maximum, with a significant reduction in by-product formation. However, the growth rate of the pflB mutant was slightly reduced compared to that of its parental strain. Comparison with similar mutants of Escherichia coli suggested that the growth defect of pflB-deficient K. pneumoniae was caused by redox imbalance rather than reduced level of intracellular acetyl coenzyme A (acetyl-CoA). From an analysis of the transcriptome, it was confirmed that the removal of pflB from K. pneumoniae significantly repressed the expression of genes involved in the formate hydrogen lyase (FHL) system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.