ArticleGenes & development2014
BR-dependent phosphorylation modulates PIF4 transcriptional activity and shapes diurnal hypocotyl growth.
Article in Genes & development, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 120 papers.
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Who cites it
120 citing papers in PubMed, 234 citations in OpenAlex.
- The BZR1-BLH1-PIF4 feedback module acts as a molecular amplifier for precise thermomorphogenesis in Arabidopsis.Nature communications · 2026Article
- Ecological Adaptation Mechanisms Underlying Successful Plant Reproduction.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Natural Variation of TaSG-D1-TaWRKY53 Module Regulates Heading Date in Wheat.Plant biotechnology journal · 2026Article
- Warm temperature modifies cell fates to reduce stomata production in Arabidopsis.The New phytologist · 2025Article
- Split-YFP-coupled interaction-dependent TurboID identifies new functions of basal cell polarity inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- A PIF-SAUR module safeguards hypocotyl elongation from ABA inhibition in the dark.Science advances · 2025Article
- Understanding the Brassinosteroid-Dependent Environmental Adaption in Brassicaceae Plants.Plants (Basel, Switzerland) · 2025Review
- Natural variation in TaERF-A1 confers semi-dwarf and lodging-resistant plant architecture in wheat.Plant communications · 2025Article
- UV-B increases active phytochrome B to suppress thermomorphogenesis and enhance UV-B stress tolerance at high temperatures.Plant communications · 2025Article
- Genome-Wide Identification of GmPIF Family and Regulatory Pathway Analysis of GmPIF3g in Different Temperature Environments.International journal of molecular sciences · 2025Article
- MPK4-mediated phosphorylation of PHYTOCHROME INTERACTING FACTOR4 controls thermosensing by regulating histone variant H2A.Z deposition.The Plant cell · 2024Article
- PHOSPHATASE 2A dephosphorylates PHYTOCHROME-INTERACTING FACTOR3 to modulate photomorphogenesis in Arabidopsis.The Plant cell · 2024Article
- RMD and Its Suppressor MAPK6 Control Root Circumnutation and Obstacle Avoidance via BR Signaling.International journal of molecular sciences · 2024Article
- LHP1 and INO80 cooperate with ethylene signaling for warm ambient temperature response by activating specific bivalent genes.Cell reports · 2024Article
- Brassinosteroid biosynthesis and signaling: Conserved and diversified functions of core genes across multiple plant species.Plant communications · 2024Review
- The lowdown on breakdown: Open questions in plant proteolysis.The Plant cell · 2024Review
- SlCPK27 cross-links SlHY5 and SlPIF4 in brassinosteroid-dependent photo- and thermo-morphogenesis in tomato.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- The phytochrome-interacting factor genes PIF1 and PIF4 are functionally diversified due to divergence of promoters and proteins.The Plant cell · 2024Article
- Methyltransferase TaSAMT1 mediates wheat freezing tolerance by integrating brassinosteroid and salicylic acid signaling.The Plant cell · 2024Article
- What is going on inside of phytochrome B photobodies?The Plant cell · 2024Review
60 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Signaling by the hormones brassinosteroid (BR) and gibberellin (GA) is critical to normal plant growth and development and is required for hypocotyl elongation in response to dark and elevated temperatures. Active BR signaling is essential for GA promotion of hypocotyl growth and suppresses the dwarf phenotype of GA mutants. Cross-talk between these hormones occurs downstream from the DELLAs, as GA-induced destabilization of these GA signaling repressors is not affected by BRs. Here we show that the light-regulated PIF4 (phytochrome-interacting factor 4) factor is a phosphorylation target of the BR signaling kinase BRASSINOSTEROID-INSENSITIVE 2 (BIN2), which marks this transcriptional regulator for proteasome degradation. Expression of a mutated PIF41A protein lacking a conserved BIN2 phosphorylation consensus causes a severe elongated phenotype and strongly up-regulated expression of the gene targets. However, PIF41A is not able to suppress the dwarf phenotype of the bin2-1 mutant with constitutive activation of this kinase. PIFs were shown to be required for the constitutive BR response of bes1-D and bzr1-1D mutants, these factors acting in an interdependent manner to promote cell elongation. Here, we show that bes1-D seedlings are still repressed by the inhibitor BRZ in the light and that expression of the nonphosphorylatable PIF41A protein makes this mutant fully insensitive to brassinazole (BRZ). PIF41A is preferentially stabilized at dawn, coinciding with the diurnal time of maximal growth. These results uncover a main role of BRs in antagonizing light signaling by inhibiting BIN2-mediated destabilization of the PIF4 factor. This regulation plays a prevalent role in timing hypocotyl elongation to late night, before light activation of phytochrome B (PHYB) and accumulation of DELLAs restricts PIF4 transcriptional activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.