ArticleActa crystallographica. Section D, Biological crystallography2014
Improved reproducibility of unit-cell parameters in macromolecular cryocrystallography by limiting dehydration during crystal mounting.
Article in Acta crystallographica. Section D, Biological crystallography, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Automated gradient equilibration of macromolecular crystals to new solution conditions.Acta crystallographica. Section F, Structural biology communications · 2025Article
- An experimental proxy of water displaceability for ligand discovery.Nature methods · 2025Article
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- MatchMaps: Non-isomorphous difference maps for X-ray crystallography.bioRxiv : the preprint server for biology · 2024Article
- Determining biomolecular structures near room temperature using X-ray crystallography: concepts, methods and future optimization.Acta crystallographica. Section D, Structural biology · 2023Article
- Integrated sample-handling and mounting system for fixed-target serial synchrotron crystallography.Acta crystallographica. Section D, Structural biology · 2021Article
- The low-cost Shifter microscope stage transforms the speed and robustness of protein crystal harvesting.Acta crystallographica. Section D, Structural biology · 2021Article
- High-throughput in situ experimental phasing.Acta crystallographica. Section D, Structural biology · 2020Article
- A comparison of gas stream cooling and plunge cooling of macromolecular crystals.Journal of applied crystallography · 2019Article
- Structural characterization of a prolyl aminodipeptidase (PepX) from Lactobacillus helveticus.Acta crystallographica. Section F, Structural biology communications · 2019Article
- A temperature-controlled cold-gas humidifier and its application to protein crystals with the humid-air and glue-coating method.Journal of applied crystallography · 2019Article
- Journey to the center of the protein: allostery from multitemperature multiconformer X-ray crystallography.Acta crystallographica. Section D, Structural biology · 2019Article
- The impact of cryosolution thermal contraction on proteins and protein crystals: volumes, conformation and order.Acta crystallographica. Section D, Structural biology · 2018Article
- Sample manipulation and data assembly for robust microcrystal synchrotron crystallography.IUCrJ · 2018Article
- Effects of protein-crystal hydration and temperature on side-chain conformational heterogeneity in monoclinic lysozyme crystals.Acta crystallographica. Section D, Structural biology · 2018Article
- A standardized technique for high-pressure cooling of protein crystals.Acta crystallographica. Section D, Structural biology · 2017Article
- Article
- A generic protocol for protein crystal dehydration using the HC1b humidity controller.Acta crystallographica. Section D, Structural biology · 2016Article
- Crystal Dehydration in Membrane Protein Crystallography.Advances in experimental medicine and biology · 2016Article
- Crystallographic phasing from weak anomalous signals.Current opinion in structural biology · 2015Review
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4 authors.
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Abstract
In macromolecular cryocrystallography unit-cell parameters can have low reproducibility, limiting the effectiveness of combining data sets from multiple crystals and inhibiting the development of defined repeatable cooling protocols. Here, potential sources of unit-cell variation are investigated and crystal dehydration during loop-mounting is found to be an important factor. The amount of water lost by the unit cell depends on the crystal size, the loop size, the ambient relative humidity and the transfer distance to the cooling medium. To limit water loss during crystal mounting, a threefold strategy has been implemented. Firstly, crystal manipulations are performed in a humid environment similar to the humidity of the crystal-growth or soaking solution. Secondly, the looped crystal is transferred to a vial containing a small amount of the crystal soaking solution. Upon loop transfer, the vial is sealed, which allows transport of the crystal at its equilibrated humidity. Thirdly, the crystal loop is directly mounted from the vial into the cold gas stream. This strategy minimizes the exposure of the crystal to relatively low humidity ambient air, improves the reproducibility of low-temperature unit-cell parameters and offers some new approaches to crystal handling and cryoprotection.
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