ArticlePloS one2014
Alfalfa Cellulose synthase gene expression under abiotic stress: a Hitchhiker's guide to RT-qPCR normalization.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 59 citations in OpenAlex.
- Coordinated physiological and molecular reprogramming by brassinosteroids improves soybean tolerance to combined salt and drought stress.Plant signaling & behavior · 2026Article
- The heat shock factor 20-HSF4-cellulose synthase A2 module regulates heat stress tolerance in maize.The Plant cell · 2024Article
- Investigating the influence of selenium and epibrassinolide on antioxidant activity, proline accumulation, and protein expression profiles in wheat plants experiencing heat and drought stress.Frontiers in plant science · 2024Article
- Physiological and gene expression responses involved in teak (Tectona grandis L.) seedlings exposed to osmotic and salt stressors.Molecular biology reports · 2023Article
- The Conserved Cysteine-Rich Secretory Protein MaCFEM85 Interacts with MsWAK16 to Activate Plant Defenses.International journal of molecular sciences · 2023Article
- Deciphering the role of SPL12 and AGL6 from a genetic module that functions in nodulation and root regeneration in Medicago sativa.Plant molecular biology · 2022Article
- SPL12 RegulatesPlants (Basel, Switzerland) · 2022Article
- Article
- How salt stress-responsive proteins regulate plant adaptation to saline conditions.Plant molecular biology · 2022Review
- Roles of Brassinosteroids in Mitigating Heat Stress Damage in Cereal Crops.International journal of molecular sciences · 2021Review
- Comparative analysis of drought-responsive and -adaptive genes in Chinese wingnut (Pterocarya stenoptera C. DC).BMC genomics · 2021Article
- Article
- Impact of abiotic stress on the regulation of cell wall biosynthesis inPlant biotechnology (Tokyo, Japan) · 2020Article
- The Roots of Plant Frost Hardiness and Tolerance.Plant & cell physiology · 2020Review
- Selection of Appropriate Reference Genes for Gene Expression Analysis under Abiotic Stresses inInternational journal of molecular sciences · 2019Article
- Genome-Wide Identification, Expression Pattern Analysis and Evolution of the Ces/Csl Gene Superfamily in Pineapple (Plants (Basel, Switzerland) · 2019Article
- Does long-term cadmium exposure influence the composition of pectic polysaccharides in the cell wall of Medicago sativa stems?BMC plant biology · 2019Article
- Study on differentially expressed genes related to defoliation traits in two alfalfa varieties based on RNA-Seq.BMC genomics · 2018Article
- Long-term cadmium exposure influences the abundance of proteins that impact the cell wall structure in Medicago sativa stems.Plant biology (Stuttgart, Germany) · 2018Article
- Identification of reference genes for RT-qPCR data normalization in Gammarus fossarum (Crustacea Amphipoda).Scientific reports · 2018Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Abiotic stress represents a serious threat affecting both plant fitness and productivity. One of the promptest responses that plants trigger following abiotic stress is the differential expression of key genes, which enable to face the adverse conditions. It is accepted and shown that the cell wall senses and broadcasts the stress signal to the interior of the cell, by triggering a cascade of reactions leading to resistance. Therefore the study of wall-related genes is particularly relevant to understand the metabolic remodeling triggered by plants in response to exogenous stresses. Despite the agricultural and economical relevance of alfalfa (Medicago sativa L.), no study, to our knowledge, has addressed specifically the wall-related gene expression changes in response to exogenous stresses in this important crop, by monitoring the dynamics of wall biosynthetic gene expression. We here identify and analyze the expression profiles of nine cellulose synthases, together with other wall-related genes, in stems of alfalfa plants subjected to different abiotic stresses (cold, heat, salt stress) at various time points (e.g. 0, 24, 72 and 96 h). We identify 2 main responses for specific groups of genes, i.e. a salt/heat-induced and a cold/heat-repressed group of genes. Prior to this analysis we identified appropriate reference genes for expression analyses in alfalfa, by evaluating the stability of 10 candidates across different tissues (namely leaves, stems, roots), under the different abiotic stresses and time points chosen. The results obtained confirm an active role played by the cell wall in response to exogenous stimuli and constitute a step forward in delineating the complex pathways regulating the response of plants to abiotic stresses.
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