ArticlePloS one2014
Identification of the microbiota in carious dentin lesions using 16S rRNA gene sequencing.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 3 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
42 citing papers in PubMed, 3 syntheses or guidelines pooled it, 73 citations in OpenAlex.
- Gene sequencing applications to combat oral-cavity related disorders: a systematic review with meta-analysis.BMC oral health · 2024Pooled it
- Meta-Analysis Using NGS Data: TheFrontiers in oral health · 2021Pooled it
- Effect of Antimicrobial Interventions on the Oral Microbiota Associated with Early Childhood Caries.Pediatric dentistryPooled it
- Topic Application of the ProbioticFrontiers in cellular and infection microbiology · 2020Trial
- Trial
- Microbial Composition of Carious Dentin and the Impact of Minimally Invasive Excavation Techniques: A Narrative Review.International journal of molecular sciences · 2026Review
- Profiling of microbes associated with chronic irreversible pulpitis using metagenomic next-generation sequencing.BMC oral health · 2025Article
- Dental caries shallow-layer microbe harvest and data display method shows taxa pre-harvest spatial positions, absolute and relative abundance and diversity related to lesion pulpal depth.Journal of oral microbiology · 2025Article
- Nepali oral microbiomes reflect a gradient of lifestyles from traditional to industrialized.Microbiome · 2024Article
- Comparative analysis of microbiome in coronal and root caries.BMC oral health · 2024Article
- A fast solution to the lasso problem with equality constraints.Journal of computational and graphical statistics : a joint publication of American Statistical Association, Institute of Mathematical Statistics, Interface Foundation of North America · 2024Article
- Overexpression of proinflammatory cytokines in dental pulp tissue and distinct bacterial microbiota in carious teeth of Mexican Individuals.Frontiers in cellular and infection microbiology · 2022Article
- Diversity of site-specific microbes of occlusal and proximal lesions in severe- early childhood caries (S-ECC).Journal of oral microbiology · 2022Article
- Periodontal Pathogens Inhabit Root Caries Lesions Extending beyond the Gingival Margin: A Next-Generation Sequencing Analysis.Microorganisms · 2021Article
- Tracking the transition to agriculture in Southern Europe through ancient DNA analysis of dental calculus.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- How Do Polymer Coatings Affect the Growth and Bacterial Population of a Biofilm Formed by Total Human Salivary Bacteria?-A Study by 16S-RNA Sequencing.Microorganisms · 2021Article
- Biodiversity ofAnimals : an open access journal from MDPI · 2021Article
- Quantification and gene expression ofThe Saudi dental journal · 2021Article
- Oral and Gut Microbial Carbohydrate-Active Enzymes Landscape in Health and Disease.Frontiers in microbiology · 2021Article
- Dynamic Observation of the Effect of Maternal Caries on the Oral Microbiota of Infants Aged 12-24 Months.Frontiers in cellular and infection microbiology · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While mutans streptococci have long been assumed to be the specific pathogen responsible for human dental caries, the concept of a complex dental caries-associated microbiota has received significant attention in recent years. Molecular analyses revealed the complexity of the microbiota with the predominance of Lactobacillus and Prevotella in carious dentine lesions. However, characterization of the dentin caries-associated microbiota has not been extensively explored in different ethnicities and races. In the present study, the bacterial communities in the carious dentin of Japanese subjects were analyzed comprehensively with molecular approaches using the16S rRNA gene. Carious dentin lesion samples were collected from 32 subjects aged 4-76 years, and the 16S rRNA genes, amplified from the extracted DNA with universal primers, were sequenced with a pyrosequencer. The bacterial composition was classified into clusters I, II, and III according to the relative abundance (high, middle, low) of Lactobacillus. The bacterial composition in cluster II was composed of relatively high proportions of Olsenella and Propionibacterium or subdominated by heterogeneous genera. The bacterial communities in cluster III were characterized by the predominance of Atopobium, Prevotella, or Propionibacterium with Streptococcus or Actinomyces. Some samples in clusters II and III, mainly related to Atopobium and Propionibacterium, were novel combinations of microbiota in carious dentin lesions and may be characteristic of the Japanese population. Clone library analysis revealed that Atopobium sp. HOT-416 and P. acidifaciens were specific species associated with dentinal caries among these genera in a Japanese population. We summarized the bacterial composition of dentinal carious lesions in a Japanese population using next-generation sequencing and found typical Japanese types with Atopobium or Propionibacterium predominating.
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Registered trials
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