ArticleInvestigative ophthalmology & visual science2014
The Zeb proteins δEF1 and Sip1 may have distinct functions in lens cells following cataract surgery.
Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- LIRTS Viewer: A Web-Based Resource to View the Transcriptional Response of Lens Epithelial Cells to Injury.Investigative ophthalmology & visual science · 2025Article
- Mefunidone Inhibits Inflammation, Oxidative Stress, and Epithelial-Mesenchymal Transition in Lens Epithelial Cells.Investigative ophthalmology & visual science · 2024Article
- Transcriptome exploration of ferroptosis-related genes in TGFβ- induced lens epithelial to mesenchymal transition during posterior capsular opacification development.BMC genomics · 2024Article
- Human lens epithelial cells induce the inflammatory response when placed into the lens capsular bag model of posterior capsular opacification.Molecular vision · 2024Article
- Modeling Cataract Surgery in Mice.Journal of visualized experiments : JoVE · 2023Article
- First Case Report of Developmental Bilateral Cataract with a Novel Mutation in theMedicina (Kaunas, Lithuania) · 2023Article
- The effect of sex on the mouse lens transcriptome.Experimental eye research · 2021Article
- The aging mouse lens transcriptome.Experimental eye research · 2021Article
- Lens Epithelial Cells Initiate an Inflammatory Response Following Cataract Surgery.Investigative ophthalmology & visual science · 2018Article
- Spatiotemporal dynamics of canonical Wnt signaling during embryonic eye development and posterior capsular opacification (PCO).Experimental eye research · 2018Article
- β1-Integrin Deletion From the Lens Activates Cellular Stress Responses Leading to Apoptosis and Fibrosis.Investigative ophthalmology & visual science · 2017Article
- Development of novel filtering criteria to analyze RNA-sequencing data obtained from the murine ocular lens during embryogenesis.Genomics data · 2014Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
purposePosterior capsular opacification (PCO), the most prevalent side effect of cataract surgery, occurs when residual lens epithelial cells (LECs) undergo fiber cell differentiation or epithelial-to-mesenchymal transition (EMT). Here, we used a murine cataract surgery model to investigate the role of the Zeb proteins, Smad interacting protein 1 (Sip1) and δ-crystallin enhancer-binding factor 1 (δEF1), during PCO.
methodsExtracapsular extraction of lens fiber cells was performed on wild-type and Sip1 knockout mice. Protein expression patterns were assessed at multiple time points after surgery using confocal immunofluorescence. βB1-Crystallin mRNA levels were measured using quantitative RT-PCR. We used Transfac searches to identify δEF1 binding sites in the βB1-crystallin promoter and transfection analysis to test the ability of δEF1 to regulate βB1-crystallin expression.
resultsδEF1, which, in other systems, can activate fibrotic genes (e.g., α-smooth muscle actin) and repress epithelial genes, upregulates by 48 hours after fiber cell removal. In culture, δEF1 repressed βB1-crystallin promoter activity, suggesting that it may also turn off lens gene expression following surgery, contributing to "fibrotic PCO" development. Sip1 also upregulates in LECs by 48 hours, but analysis of Sip1 knockout lenses demonstrated that Sip1 does not play a major role in EMT or fiber cell differentiation after surgery. However, Sip1 knockout LECs do express the ectodermal marker keratin 8, suggesting that Sip1 may limit the reprogramming of residual LECs to an embryonic state.
conclusionsZeb transcription factors likely play important, but distinct roles in PCO development after cataract surgery.
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