Evidence map›Paper›PMID 25082855›Full record

ArticleThe Plant cell2014

Genetical and comparative genomics of Brassica under altered Ca supply identifies Arabidopsis Ca-transporter orthologs.

Neil S Graham, John P Hammond, Artem Lysenko, Sean Mayes, Seosamh O Lochlainn, Bego Blasco, Helen C Bowen, Chris J Rawlings, Juan J Rios, Susan Welham and 5 more

Abstract read
In one paragraph

Article in The Plant cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
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  7. Review
  8. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Neil S GrahamPlant and Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, United Kingdom.
John P HammondSchool of Agriculture, Policy, and Development, University of Reading, Earley Gate, Whiteknights, Reading RG6 6AR, United Kingdom.
Artem LysenkoComputational and Systems Biology Department, Rothamsted Research, West Common, Harpenden AL5 2JQ, United Kingdom.
Sean MayesCrops for the Future Research Centre, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia.
Seosamh O LochlainnPlant and Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, United Kingdom.
Bego BlascoPlant and Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, United Kingdom.
Helen C BowenWarwick HRI, University of Warwick, Wellesbourne CV35 9EF, United Kingdom.
Chris J RawlingsComputational and Systems Biology Department, Rothamsted Research, West Common, Harpenden AL5 2JQ, United Kingdom.
Juan J RiosPlant and Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, United Kingdom.
Susan WelhamComputational and Systems Biology Department, Rothamsted Research, West Common, Harpenden AL5 2JQ, United Kingdom.
Pierre W C CarionComputational and Systems Biology Department, Rothamsted Research, West Common, Harpenden AL5 2JQ, United Kingdom.
Lionel X DupuyJames Hutton Institute, Invergowrie, Dundee DD2 5DA, United Kingdom.
Graham J KingSouthern Cross Plant Science, Southern Cross University, Lismore, New South Wales 2480, Australia.
Philip J WhiteJames Hutton Institute, Invergowrie, Dundee DD2 5DA, United Kingdom College of Science, King Saud University, Riyadh 11451, Kingdom of Saudi Arabia.
Martin R BroadleyPlant and Crop Sciences Division, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, United Kingdom martin.broadley@nottingham.ac.uk.

Funding

Biotechnology and Biological Sciences Research Council BB-G013969-1Biotechnology and Biological Sciences Research Council BB/G013969/1Biotechnology and Biological Sciences Research Council BB/G014159/1Biotechnology and Biological Sciences Research Council BB/G015716/1
6 · The paper itself

Abstract

Although Ca transport in plants is highly complex, the overexpression of vacuolar Ca(2+) transporters in crops is a promising new technology to improve dietary Ca supplies through biofortification. Here, we sought to identify novel targets for increasing plant Ca accumulation using genetical and comparative genomics. Expression quantitative trait locus (eQTL) mapping to 1895 cis- and 8015 trans-loci were identified in shoots of an inbred mapping population of Brassica rapa (IMB211 × R500); 23 cis- and 948 trans-eQTLs responded specifically to altered Ca supply. eQTLs were screened for functional significance using a large database of shoot Ca concentration phenotypes of Arabidopsis thaliana. From 31 Arabidopsis gene identifiers tagged to robust shoot Ca concentration phenotypes, 21 mapped to 27 B. rapa eQTLs, including orthologs of the Ca(2+) transporters At-CAX1 and At-ACA8. Two of three independent missense mutants of BraA.cax1a, isolated previously by targeting induced local lesions in genomes, have allele-specific shoot Ca concentration phenotypes compared with their segregating wild types. BraA.CAX1a is a promising target for altering the Ca composition of Brassica, consistent with prior knowledge from Arabidopsis. We conclude that multiple-environment eQTL analysis of complex crop genomes combined with comparative genomics is a powerful technique for novel gene identification/prioritization.

Indexed as

ArabidopsisBrassicaCalciumCation Transport ProteinsChromosome MappingCrops, AgriculturalGene-Environment InteractionGene Expression Regulation, PlantGenome, PlantGenomicsMutation, MissensePhenotypePlant LeavesPlant ProteinsPlants, Genetically ModifiedPlant ShootsCalciumCation Transport ProteinsPlant Proteins

Identifiers

PMID25082855
PMCPMC4145116

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.