ArticleCurrent protocols in microbiology2014
Molecular Typing of Borrelia burgdorferi.
Article in Current protocols in microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Resistance ofPathogens (Basel, Switzerland) · 2026Article
- Fast and efficient Borrelia chromosome recovery from tick samples using whole-genome amplification.Cell reports methods · 2026Article
- Machine learning-driven identification of virulence determinants in Borrelia burgdorferi associated with human dissemination.PLoS computational biology · 2026Article
- Influence of tick age and land-use on Borrelia burgdorferi s.l. in Ixodes ricinus ticks from the Swabian Alb, Germany.Parasites & vectors · 2025Article
- Article
- Development, Optimization, and Validation of a Quantitative PCR Assay forPathogens (Basel, Switzerland) · 2024Article
- The first molecular record ofPathogens and global health · 2024Article
- Whole-genome sequencing of Western CanadianMicrobial genomics · 2024Article
- Development of a sequence-basedMicrobial genomics · 2024Article
- Molecular Typing on Human Blood Reveals theInfection & chemotherapy · 2023Article
- Whole genome sequencing of human Borrelia burgdorferi isolates reveals linked blocks of accessory genome elements located on plasmids and associated with human dissemination.PLoS pathogens · 2023Article
- Whole genome sequencing ofbioRxiv : the preprint server for biology · 2023Article
- Diversity of Borrelia burgdorferi sensu lato in ticks and small mammals from different habitats.Parasites & vectors · 2022Article
- Article
- First report of Borrelia burgdorferi sensu stricto detection in a commune genospecies in Apodemus agrarius in Gwangju, South Korea.Scientific reports · 2021Article
- Recent Progress in Lyme Disease and Remaining Challenges.Frontiers in medicine · 2021Review
- Lyme Disease Frontiers: ReconcilingPathogens (Basel, Switzerland) · 2019Review
- The Density of the Lyme Disease Vector,Northeastern naturalist · 2019Article
- Long-term trends of tick-borne pathogens in regard to small mammal and tick populations from Saxony, Germany.Parasites & vectors · 2019Article
- Metagenomic 16S rRNA gene sequencing survey of Borrelia species in Irish samples of Ixodes ricinus ticks.PloS one · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Borrelia burgdorferi sensu lato is a group of spirochetes belonging to the genus Borrelia in the family of Spirochaetaceae. The spirochete is transmitted between reservoirs and hosts by ticks of the family Ixodidae. Infection with B. burgdorferi in humans causes Lyme disease or Lyme borreliosis. Currently, 20 Lyme disease-associated Borrelia species and more than 20 relapsing fever-associated Borrelia species have been described. Identification and differentiation of different Borrelia species and strains is largely dependent on analyses of their genetic characteristics. A variety of molecular techniques have been described for Borrelia isolate speciation, molecular epidemiology, and pathogenicity studies. In this unit, we focus on three basic protocols, PCR-RFLP-based typing of the rrs-rrlA and rrfA-rrlB ribosomal spacer, ospC typing, and MLST. These protocols can be employed alone or in combination for characterization of B. burgdorferi isolates or directly on uncultivated organisms in ticks, mammalian host reservoirs, and human clinical specimens.
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