ArticleThe Journal of infectious diseases2015
Editing the Plasmodium vivax genome, using zinc-finger nucleases.
Article in The Journal of infectious diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
- HaloPROTAC3 does not trigger the degradation of the halotagged parasitophorous vacuole membrane protein UIS4 during Plasmodium liver stage development.Scientific reports · 2025Article
- Transfection Models to InvestigatePathogens (Basel, Switzerland) · 2023Review
- Integrative Genetic Manipulation of Plasmodium cynomolgi Reveals Multidrug Resistance-1 Y976F Associated With Increased In Vitro Susceptibility to Mefloquine.The Journal of infectious diseases · 2023Article
- Culture and transfection: Two major bottlenecks in understandingFrontiers in microbiology · 2023Review
- Advances in the Genetic Manipulation of Nosema bombycis.Experientia supplementum (2012) · 2022Article
- Activity of Plasmodium vivax promoter elements in Plasmodium knowlesi, and a centromere-containing plasmid that expresses NanoLuc throughout the parasite life cycle.Malaria journal · 2021Article
- Genomic and Genetic Approaches to Studying Antimalarial Drug Resistance and Plasmodium Biology.Trends in parasitology · 2021Review
- Genetic Manipulation of Non-Falciparum Human Malaria Parasites.Frontiers in cellular and infection microbiology · 2021Review
- Antimalarial Drug Resistance and Implications for the WHO Global Technical Strategy.Current epidemiology reports · 2021Review
- Modeling Relapsing Malaria: Emerging Technologies to Study Parasite-Host Interactions in the Liver.Frontiers in cellular and infection microbiology · 2020Review
- Genome Editing ofmSphere · 2019Article
- Validation of Plasmodium vivax centromere and promoter activities using Plasmodium yoelii.PloS one · 2019Article
- Guide RNA selection for CRISPR-Cas9 transfections in Plasmodium falciparum.International journal for parasitology · 2018Article
- Article
- Advancing Research Models and Technologies to Overcome Biological Barriers to Plasmodium vivax Control.Trends in parasitology · 2018Review
- Host Cell Tropism and Adaptation of Blood-Stage Malaria Parasites: Challenges for Malaria Elimination.Cold Spring Harbor perspectives in medicine · 2017Review
- Selective sweep suggests transcriptional regulation may underlie Plasmodium vivax resilience to malaria control measures in Cambodia.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Transfected Babesia bovis Expressing a Tick GST as a Live Vector Vaccine.PLoS neglected tropical diseases · 2016Article
- Advances in molecular genetic systems in malaria.Nature reviews. Microbiology · 2015Review
- Towards genome-wide experimental genetics in the in vivo malaria model parasite Plasmodium berghei.Pathogens and global health · 2015Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Plasmodium vivax is a major cause of malaria morbidity worldwide yet has remained genetically intractable. To stably modify this organism, we used zinc-finger nucleases (ZFNs), which take advantage of homology-directed DNA repair mechanisms at the site of nuclease action. Using ZFNs specific to the gene encoding P. vivax dihydrofolate reductase (pvdhfr), we transfected blood specimens from Saimiri boliviensis monkeys infected with the pyrimethamine (Pyr)-susceptible Chesson strain with a ZFN plasmid carrying a Pyr-resistant mutant pvdhfr sequence. We obtained Pyr-resistant parasites in vivo that carried mutant pvdhfr and additional silent mutations designed to confirm editing. These results herald the era of stable P. vivax genetic modifications.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.