ArticleJournal of the American Chemical Society2014
Probing the phosphopantetheine arm conformations of acyl carrier proteins using vibrational spectroscopy.
Article in Journal of the American Chemical Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Article
- Article
- Genome-wide analysis of FATA associated with drought tolerance in tetraploid potato (Frontiers in plant science · 2026Article
- AMOEBA Force Field Predicts Accurate Hydrogen Bond Counts of Nitriles in SNase by Revealing Water-Protein Interaction in Vibrational Absorption Frequencies.The journal of physical chemistry. B · 2023Article
- Heterologous Expression, Purification, and Characterization of Type II Polyketide Synthase Acyl Carrier Proteins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Structural Characterization of an ACP fromInternational journal of molecular sciences · 2020Article
- Colorimetric Assay Reports on Acyl Carrier Protein Interactions.Scientific reports · 2019Article
- Trapping interactions between catalytic domains and carrier proteins of modular biosynthetic enzymes with chemical probes.Natural product reports · 2018Review
- Conformation-specific detection of calmodulin binding using the unnatural amino acid p-azido-phenylalanine (AzF) as an IR-sensor.Structural dynamics (Melville, N.Y.) · 2018Article
- Cyanylated Cysteine Reports Site-Specific Changes at Protein-Protein-Binding Interfaces Without Perturbation.Biochemistry · 2018Article
- Conformational Ensembles of Calmodulin Revealed by Nonperturbing Site-Specific Vibrational Probe Groups.The journal of physical chemistry. A · 2018Article
- Metabolic network model guided engineering ethylmalonyl-CoA pathway to improve ascomycin production in Streptomyces hygroscopicus var. ascomyceticus.Microbial cell factories · 2017Article
- Acyl Carrier Protein Cyanylation Delivers a Ketoacyl Synthase-Carrier Protein Cross-Link.Biochemistry · 2017Article
- Simple method to introduce an ester infrared probe into proteins.Protein science : a publication of the Protein Society · 2017Article
- Sticky swinging arm dynamics: studies of an acyl carrier protein domain from the mycolactone polyketide synthase.The Biochemical journal · 2016Article
- Recognition of extended linear and cyclised polyketide mimics by a type II acyl carrier protein.Chemical science · 2016Article
- Reinvigorating natural product combinatorial biosynthesis with synthetic biology.Nature chemical biology · 2015Article
- Using modern tools to probe the structure-function relationship of fatty acid synthases.Chembiochem : a European journal of chemical biology · 2015Review
- Visualizing the chain-flipping mechanism in fatty-acid biosynthesis.Angewandte Chemie (International ed. in English) · 2014Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Acyl carrier proteins (ACPs) are universal and highly conserved domains central to both fatty acid and polyketide biosynthesis. These proteins tether reactive acyl intermediates with a swinging 4'-phosphopantetheine (Ppant) arm and interact with a suite of catalytic partners during chain transport and elongation while stabilizing the growing chain throughout the biosynthetic pathway. The flexible nature of the Ppant arm and the transient nature of ACP-enzyme interactions impose a major obstacle to obtaining structural information relevant to understanding polyketide and fatty acid biosynthesis. To overcome this challenge, we installed a thiocyanate vibrational spectroscopic probe on the terminal thiol of the ACP Ppant arm. This site-specific probe successfully reported on the local environment of the Ppant arm of two ACPs previously characterized by solution NMR, and was used to determine the solution exposure of the Ppant arm of an ACP from 6-deoxyerythronolide B synthase (DEBS). Given the sensitivity of the probe's CN stretching band to conformational distributions resolved on the picosecond time scale, this work lays a foundation for observing the dynamic action-related structural changes of ACPs using vibrational spectroscopy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.