ArticleBMC genetics2014
Transcriptome sequencing of transgenic poplar (Populus × euramericana 'Guariento') expressing multiple resistance genes.
Article in BMC genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed.
- Characterization of two glycosyltransferases that modulate withanolide biosynthesis and defense in Withania somnifera.Molecular biology reports · 2025Article
- The transcriptional regulation of a putative hemicellulose gene, PtrPARVUS2 in poplar.Scientific reports · 2024Article
- Ectopic expression of a truncated NLR gene from wildFrontiers in plant science · 2024Article
- Transcriptomic and Physiological Analysis Reveals Genes Associated with Drought Stress Responses inPlants (Basel, Switzerland) · 2023Article
- Elevated COPlants (Basel, Switzerland) · 2022Article
- Integrative RNA-Seq analysis ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Transcriptomic Analysis of Mature Transgenic Poplar Expressing the Transcription FactorFrontiers in bioengineering and biotechnology · 2022Article
- Overexpression of transcription factor SlWRKY28 improved the tolerance of Populus davidiana × P. bolleana to alkaline salt stress.BMC genetics · 2020Article
- Comparative Metabolomic Analysis of the Cambium Tissue of Non-transgenic and Multi-Gene Transgenic Poplar (Frontiers in plant science · 2018Article
- Exploring genes involved in benzoic acid biosynthesis in the Populus davidiana transcriptome and their transcriptional activity upon methyl jasmonate treatment.Journal of chemical ecology · 2017Article
- Analysis of transcription factors among differentially expressed genes induced by drought stress in Populus davidiana.3 Biotech · 2017Article
- Transcriptome analysis of the tea oil camellia (Camellia oleifera) reveals candidate drought stress genes.PloS one · 2017Article
- Proteome quantification of cotton xylem sap suggests the mechanisms of potassium-deficiency-induced changes in plant resistance to environmental stresses.Scientific reports · 2016Article
- Ectopic overexpression of WsSGTL1, a sterol glucosyltransferase gene in Withania somnifera, promotes growth, enhances glycowithanolide and provides tolerance to abiotic and biotic stresses.Plant cell reports · 2016Article
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Authors and funding
9 authors.
Funding
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Abstract
backgroundTransgenic poplar (Populus × euramericana 'Guariento') plants harboring five exogenous, stress-related genes exhibit increased tolerance to multiple stresses including drought, salt, waterlogging, and insect feeding, but the complex mechanisms underlying stress tolerance in these plants have not been elucidated. Here, we analyzed the differences in the transcriptomes of the transgenic poplar line D5-20 and the non-transgenic line D5-0 using high-throughput transcriptome sequencing techniques and elucidated the functions of the differentially expressed genes using various functional annotation methods.
resultsWe generated 11.80 Gb of sequencing data containing 63, 430, 901 sequences, with an average length of 200 bp. The processed sequences were mapped to reference genome sequences of Populus trichocarpa. An average of 62.30% and 61.48% sequences could be aligned with the reference genomes for D5-20 and D5-0, respectively. We detected 11,352 (D5-20) and 11,372 expressed genes (D5-0), 7,624 (56.61%; D5-20) and 7,453 (65.54%; D5-0) of which could be functionally annotated. A total of 782 differentially expressed genes in D5-20 were identified compared with D5-0, including 628 up-regulated and 154 down-regulated genes. In addition, 196 genes with putative functions related to stress responses were also annotated. Gene Ontology (GO) analysis revealed that 346 differentially expressed genes are mainly involved in 67 biological functions, such as DNA binding and nucleus. KEGG annotation revealed that 36 genes (21 up-regulated and 15 down-regulated) were enriched in 51 biological pathways, 9 of which are linked to glucose metabolism. KOG functional classification revealed that 475 genes were enriched in 23 types of KOG functions.
conclusionThese results suggest that the transferred exogenous genes altered the expression of stress (biotic and abiotic) response genes, which were distributed in different metabolic pathways and were linked to some extent. Our results provide a theoretic basis for investigating the functional mechanisms of exogenous genes in transgenic plants.
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