ArticleMarine biotechnology (New York, N.Y.)2015
De novo characterisation of the greenlip abalone transcriptome (Haliotis laevigata) with a focus on the heat shock protein 70 (HSP70) family.
Article in Marine biotechnology (New York, N.Y.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 48 citations in OpenAlex.
- Proteomics and phosphoproteomics reveal novel proteins involved inFrontiers in chemistry · 2024Article
- Whole genome resequencing reveals signatures of rapid selection in a virus-affected commercial fishery.Molecular ecology · 2022Article
- Phylogenetic annotation ofmicroPublication biology · 2022Article
- Transcriptomic responses to thermal stress in hybrid abalone (Frontiers in genetics · 2022Article
- Investigating the biochemical effects of heat stress and sample quenching approach on the metabolic profiling of abalone (Haliotis iris).Metabolomics : Official journal of the Metabolomic Society · 2021Article
- Immunity-related genes and signaling pathways under hypoxic stresses in Haliotis diversicolor: a transcriptome analysis.Scientific reports · 2019Article
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- RNA Sequencing Analysis to Capture the Transcriptome Landscape during Tenderization in Sea CucumberMolecules (Basel, Switzerland) · 2019Article
- Best Foot Forward: Nanopore Long Reads, Hybrid Meta-Assembly, and Haplotig Purging Optimizes the First Genome Assembly for the Southern Hemisphere Blacklip Abalone (Frontiers in genetics · 2019Article
- Gonadal Transcriptome Analysis of Pacific Abalone Haliotis discus discus: Identification of Genes Involved in Germ Cell Development.Marine biotechnology (New York, N.Y.) · 2018Article
- In-depth proteomic analyses ofProteome science · 2018Article
- Different Transcriptomic Responses to Thermal Stress in Heat-Tolerant and Heat-Sensitive Pacific Abalones Indicated by Cardiac Performance.Frontiers in physiology · 2018Article
- Transcriptomic Analysis Reveals Insights on Male Infertility inFrontiers in physiology · 2018Article
- Epipodial Tentacle Gene Expression and Predetermined Resilience to Summer Mortality in the Commercially Important Greenlip Abalone, Haliotis laevigata.Marine biotechnology (New York, N.Y.) · 2017Article
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Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Abalone (Haliotis) are economically important molluscs for fisheries and aquaculture industries worldwide. Despite this, genomic resources for abalone and molluscs are still limited. Here we present a description and functional annotation of the greenlip abalone (Haliotis laevigata) transcriptome. We present a focused analysis on the heat shock protein 70 (HSP70) family of genes with putative functions affecting temperature stress and immunity. A total of ~38 million paired end Illumina reads were obtained, resulting in a Trinity assembly of 222,172 contigs with minimum length of 200 base pairs and maximum length of 33 kilobases. The 20,702 contigs were annotated with gene descriptions by BLAST. We created a program to maximise the number of functionally annotated genes, and over 10,000 contigs were assigned Gene ontologies (GO terms). By using CateGOrizer, immunity related GO terms for stressors such as heat, hypoxia, oxidative stress and wounding received the highest counts. Twenty-six contigs with homology to the HSP70 family of genes were identified. Ninety-one putative single-nucleotide polymorphisms were observed in the abalone HSP70 contigs. Eleven of these were considered non-synonymous. The annotated transcriptome described in this study will be a useful basis for future work investigating the genetic response of abalone to stress.
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