ArticlePloS one2014
Multiplicity of 5' cap structures present on short RNAs.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Application of Mammalian Nudix Enzymes to Capped RNA Analysis.Pharmaceuticals (Basel, Switzerland) · 2024Review
- A systems-level mass spectrometry-based technique for accurate and sensitive quantification of the RNA cap epitranscriptome.Nature protocols · 2023Review
- A Novel Tissue Atlas and Online Tool for the Interrogation of Small RNA Expression in Human Tissues and Biofluids.Frontiers in cell and developmental biology · 2022Article
- Comprehensive determination of transcription start sites derived from all RNA polymerases using ReCappable-seq.Genome research · 2022Article
- Up-to-date on the evidence linking miRNA-related epitranscriptomic modifications and disease settings. Can these modifications affect cross-kingdom regulation?RNA biology · 2021Article
- 5´XP sRNA-seq: efficient identification of transcripts with and without 5´ phosphorylation reveals evolutionary conserved small RNA.RNA biology · 2021Article
- CPA-seq reveals small ncRNAs with methylated nucleosides and diverse termini.Cell discovery · 2021Article
- CAP-MAP: cap analysis protocol with minimal analyte processing, a rapid and sensitive approach to analysing mRNA cap structures.Open biology · 2020Article
- Quantifying the RNA cap epitranscriptome reveals novel caps in cellular and viral RNA.Nucleic acids research · 2019Article
- Small RNA inhibits infection by downy mildew pathogen Hyaloperonospora arabidopsidis.Molecular plant pathology · 2019Article
- The cellular landscape of mid-size noncoding RNA.Wiley interdisciplinary reviews. RNA · 2019Review
- The yeast scavenger decapping enzyme DcpS and its application for in vitro RNA recapping.Scientific reports · 2019Article
- mRNA cap regulation in mammalian cell function and fate.Biochimica et biophysica acta. Gene regulatory mechanisms · 2019Review
- A recap of RNA recapping.Wiley interdisciplinary reviews. RNA · 2019Review
- Transcriptional interference by small transcripts in proximal promoter regions.Nucleic acids research · 2018Article
- Regulation and function of CMTR1-dependent mRNA cap methylation.Wiley interdisciplinary reviews. RNA · 2017Review
- Low-cell-number, single-tube amplification (STA) of total RNA revealed transcriptome changes from pluripotency to endothelium.BMC biology · 2017Article
- Putative RNA-directed adaptive mutations in cancer evolution.Transcription · 2016Article
- mRNA capping: biological functions and applications.Nucleic acids research · 2016Review
- Precursors of tRNAs are stabilized by methylguanosine cap structures.Nature chemical biology · 2016Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Most RNA molecules are co- or post-transcriptionally modified to alter their chemical and functional properties to assist in their ultimate biological function. Among these modifications, the addition of 5' cap structure has been found to regulate turnover and localization. Here we report a study of the cap structure of human short (<200 nt) RNAs (sRNAs), using sequencing of cDNA libraries prepared by enzymatic pretreatment of the sRNAs with cap sensitive-specificity, thin layer chromatographic (TLC) analyses of isolated cap structures and mass spectrometric analyses for validation of TLC analyses. Processed versions of snoRNAs and tRNAs sequences of less than 50 nt were observed in capped sRNA libraries, indicating additional processing and recapping of these annotated sRNAs biotypes. We report for the first time 2,7 dimethylguanosine in human sRNAs cap structures and surprisingly we find multiple type 0 cap structures (mGpppC, 7mGpppG, GpppG, GpppA, and 7mGpppA) in RNA length fractions shorter than 50 nt. Finally, we find the presence of additional uncharacterized cap structures that wait determination by the creation of needed reference compounds to be used in TLC analyses. These studies suggest the existence of novel biochemical pathways leading to the processing of primary and sRNAs and the modifications of their RNA 5' ends with a spectrum of chemical modifications.
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