ArticleJournal of visualized experiments : JoVE2014
In vitro pancreas organogenesis from dispersed mouse embryonic progenitors.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Permeability-driven pressure and cell proliferation control lumen morphogenesis in pancreatic organoids.Nature cell biology · 2026Article
- Generic comparison of lumen nucleation and fusion in epithelial organoids with and without hydrostatic pressure.Nature communications · 2025Article
- Pancreatic stem cells originate during the pancreatic progenitor developmental stage.Frontiers in cell and developmental biology · 2025Article
- Long-term feeder-free culture of human pancreatic progenitors on fibronectin or matrix-free polymer potentiates β cell differentiation.Stem cell reports · 2022Article
- Improved Differentiation of hESC-Derived Pancreatic Progenitors by Using Human Fetal Pancreatic Mesenchymal Cells in a Micro-scalable Three-Dimensional Co-culture System.Stem cell reviews and reports · 2022Article
- Chick fetal organ spheroids as a model to study development and disease.BMC molecular and cell biology · 2021Article
- Recent advances in organoid development and applications in disease modeling.Biochimica et biophysica acta. Reviews on cancer · 2021Review
- Copper Transport and Disease: What Can We Learn from Organoids?Annual review of nutrition · 2019Review
- Neurogenin3 phosphorylation controls reprogramming efficiency of pancreatic ductal organoids into endocrine cells.Scientific reports · 2018Article
- Deconstructing the principles of ductal network formation in the pancreas.PLoS biology · 2018Article
- Generation of Scaffold-free, Three-dimensional Insulin Expressing Pancreatoids from Mouse Pancreatic Progenitors In Vitro.Journal of visualized experiments : JoVE · 2018Article
- Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling.PLoS biology · 2017Article
- Lung Organoids and Their Use To Study Cell-Cell Interaction.Current pathobiology reports · 2017Review
- Stem Cells in Neurotoxicology/Developmental Neurotoxicology: Current Scenario and Future Prospects.Molecular neurobiology · 2016Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The pancreas is an essential organ that regulates glucose homeostasis and secretes digestive enzymes. Research on pancreas embryogenesis has led to the development of protocols to produce pancreatic cells from stem cells (1). The whole embryonic organ can be cultured at multiple stages of development (2-4). These culture methods have been useful to test drugs and to image developmental processes. However the expansion of the organ is very limited and morphogenesis is not faithfully recapitulated since the organ flattens. We propose three-dimensional (3D) culture conditions that enable the efficient expansion of dissociated mouse embryonic pancreatic progenitors. By manipulating the composition of the culture medium it is possible to generate either hollow spheres, mainly composed of pancreatic progenitors expanding in their initial state, or, complex organoids which progress to more mature expanding progenitors and differentiate into endocrine, acinar and ductal cells and which spontaneously self-organize to resemble the embryonic pancreas. We show here that the in vitro process recapitulates many aspects of natural pancreas development. This culture system is suitable to investigate how cells cooperate to form an organ by reducing its initial complexity to few progenitors. It is a model that reproduces the 3D architecture of the pancreas and that is therefore useful to study morphogenesis, including polarization of epithelial structures and branching. It is also appropriate to assess the response to mechanical cues of the niche such as stiffness and the effects on cell´s tensegrity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.