ArticleJournal of virology2014
Engineering recombinant Orsay virus directly in the metazoan host Caenorhabditis elegans.
Article in Journal of virology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 31 citations in OpenAlex.
- Species-specific barriers restrict virus spillover potential across the Caenorhabditis genus.PLoS biology · 2026Article
- Characterizing the role of RSD-6 in the biogenesis of virus-derived small interfering RNAs and the modulation of viral pathogenesis.Journal of virology · 2026Article
- Viral RNA pUGylation promotes antiviral immunity inJournal of virology · 2025Article
- ATG-3 limits Orsay virus infection in C. elegans and regulates collagen pathways.PLoS pathogens · 2025Article
- Orsay virus variants isolated from wildMicrobiology resource announcements · 2025Article
- Characterizing the role of RSD-6 in the biogenesis of virus-derived small interfering RNAs and the modulation of viral pathogenesis.bioRxiv : the preprint server for biology · 2025Article
- Insights into Virus-Host Interactions: Lessons from Caenorhabditis elegans-Orsay Virus Model.Current medical science · 2025Review
- Natural variation in infection specificity of Caenorhabditis briggsae isolates by two RNA viruses.PLoS pathogens · 2024Article
- Collagen and actin network mediate antiviral immunity against Orsay virus in C. elegans intestinal cells.PLoS pathogens · 2024Article
- Orsay Virus Infection of Caenorhabditis elegans Is Modulated by Zinc and Dependent on Lipids.Journal of virology · 2022Article
- The Dietary Restriction-Like GeneJournal of virology · 2019Article
- Transgene-Assisted Genetic Screen IdentifiesJournal of virology · 2018Article
- Orsay δ Protein Is Required for Nonlytic Viral Egress.Journal of virology · 2018Article
- Caenorhabditis elegans as an Emerging Model for Virus-Host Interactions.Journal of virology · 2017Review
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- Expression of hepatitis B virus surface antigens induces defective gonad phenotypes in Caenorhabditis elegans.World journal of virology · 2017Article
- Structure of a pentameric virion-associated fiber with a potential role in Orsay virus entry to host cells.PLoS pathogens · 2017Article
- An expressed, endogenous Nodavirus-like element captured by a retrotransposon in the genome of the plant parasitic nematode Bursaphelenchus xylophilus.Scientific reports · 2016Article
- Fruits of virus discovery: new pathogens and new experimental models.Journal of virology · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The recent identification of Orsay virus, the first virus that is capable of naturally infecting Caenorhabditis elegans, provides a unique opportunity to explore host-virus interaction studies in this invaluable model organism. A key feature of this system is the robust genetic tractability of the host, C. elegans, which would ideally be complemented by the ability to genetically manipulate Orsay virus in parallel. To this end, we developed a plasmid-based reverse genetics system for Orsay virus by creating transgenic C. elegans strains harboring Orsay virus cDNAs. Both wild-type and mutant Orsay viruses, including a FLAG epitope-tagged recombinant Orsay virus, were generated by use of the reverse genetics system. This is the first plasmid-based virus reverse genetics system in the metazoan C. elegans. The Orsay virus reverse genetics we established will serve as a fundamental tool in host-virus interaction studies in the model organism C. elegans. Importance: To date, Orsay virus is the first and the only identified virus capable of naturally infecting Caenorhabditis elegans. C. elegans is a simple multicellular model organism that mimics many fundamental features of human biology and has been used to define many biological properties conserved through evolution. Thus, the Orsay virus-C. elegans infection system provides a unique opportunity to study host-virus interactions. In order to take maximal advantage of this system, the ability to genetically engineer mutant forms of Orsay virus would be highly desirable. Most efforts to engineer viruses have been done with cultured cells. Here we describe the creation of mutant viruses directly in the multicellular organism C. elegans without the use of cell culture. We engineered a virus expressing a genetically tagged protein that could be detected in C. elegans. This provides proof of concept for modifying Orsay virus, which will greatly facilitate studies in this experimental system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.