ArticlePLoS neglected tropical diseases2014
An integrated lab-on-chip for rapid identification and simultaneous differentiation of tropical pathogens.
Article in PLoS neglected tropical diseases, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed.
- Etiology of acute febrile illness in the peruvian amazon as determined by modular formatted quantitative PCR: a protocol for RIVERA, a health facility-based case-control study.BMC public health · 2023Article
- Fully automated point-of-care differential diagnosis of acute febrile illness.PLoS neglected tropical diseases · 2021Article
- Recent advances in lab-on-a-chip technologies for viral diagnosis.Biosensors & bioelectronics · 2020Review
- Silicon µPCR Chip for Forensic STR Profiling with Hybeacon Probe Melting Curves.Scientific reports · 2019Article
- Diagnostic tools for tackling febrile illness and enhancing patient management.Microelectronic engineering · 2018Article
- Searching for the proverbial needle in a haystack: advances in mosquito-borne arbovirus surveillance.Parasites & vectors · 2018Review
- Converging Human and Malaria Vector Diagnostics with Data Management towards an Integrated Holistic One Health Approach.International journal of environmental research and public health · 2018Review
- Genotyping of KRAS Mutational Status by the In-Check Lab-on-Chip Platform.Sensors (Basel, Switzerland) · 2018Article
- Progress and Challenges towards Point-of-Care Diagnostic Development for Dengue.Journal of clinical microbiology · 2017Review
- A Novel Pan-BioMed research international · 2017Article
- Development of Vaccines for Chikungunya Fever.The Journal of infectious diseases · 2016Review
- Chikungunya Virus Infection: An Update on Joint Manifestations and Management.Rambam Maimonides medical journal · 2016Review
- The Point-of-Care Laboratory in Clinical Microbiology.Clinical microbiology reviews · 2016Review
- Novel molecular diagnostic tools for malaria elimination: a review of options from the point of view of high-throughput and applicability in resource limited settings.Malaria journal · 2016Review
- Lab-on-Chip-Based Platform for Fast Molecular Diagnosis of Multidrug-Resistant Tuberculosis.Journal of clinical microbiology · 2015Article
- Advances in genetics and genomics: use and limitations in achieving malaria elimination goals.Pathogens and global health · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tropical pathogens often cause febrile illnesses in humans and are responsible for considerable morbidity and mortality. The similarities in clinical symptoms provoked by these pathogens make diagnosis difficult. Thus, early, rapid and accurate diagnosis will be crucial in patient management and in the control of these diseases. In this study, a microfluidic lab-on-chip integrating multiplex molecular amplification and DNA microarray hybridization was developed for simultaneous detection and species differentiation of 26 globally important tropical pathogens. The analytical performance of the lab-on-chip for each pathogen ranged from 102 to 103 DNA or RNA copies. Assay performance was further verified with human whole blood spiked with Plasmodium falciparum and Chikungunya virus that yielded a range of detection from 200 to 4×105 parasites, and from 250 to 4×107 PFU respectively. This lab-on-chip was subsequently assessed and evaluated using 170 retrospective patient specimens in Singapore and Thailand. The lab-on-chip had a detection sensitivity of 83.1% and a specificity of 100% for P. falciparum; a sensitivity of 91.3% and a specificity of 99.3% for P. vivax; a positive 90.0% agreement and a specificity of 100% for Chikungunya virus; and a positive 85.0% agreement and a specificity of 100% for Dengue virus serotype 3 with reference methods conducted on the samples. Results suggested the practicality of an amplification microarray-based approach in a field setting for high-throughput detection and identification of tropical pathogens.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.