Evidence map›Paper›PMID 25078058›Full record

ArticleScientific reports2014

Carbohydrate-based ice recrystallization inhibitors increase infectivity and thermostability of viral vectors.

Shahrokh M Ghobadloo, Anna K Balcerzak, Ana Gargaun, Darija Muharemagic, Gleb G Mironov, Chantelle J Capicciotti, Jennie G Briard, Robert N Ben, Maxim V Berezovski

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.2field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Aptamer-facilitated cryoprotection of viruses.ACS medicinal chemistry letters · 2014
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Shahrokh M GhobadlooDepartment of Chemistry, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada.
Anna K BalcerzakDepartment of Chemistry, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada.
Ana GargaunDepartment of Chemistry, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada.
Darija MuharemagicDepartment of Chemistry, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada.
Gleb G MironovDepartment of Chemistry, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada.
Chantelle J CapicciottiDepartment of Chemistry, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada.
Jennie G BriardDepartment of Chemistry, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada.
Robert N BenDepartment of Chemistry, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada.
Maxim V BerezovskiDepartment of Chemistry, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada.
University of Ottawa · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The inability of vaccines to retain sufficient thermostability has been an obstacle to global vaccination programs. To address this major limitation, we utilized carbohydrate-based ice recrystallization inhibitors (IRIs) to eliminate the cold chain and stabilize the potency of Vaccinia virus (VV), Vesicular Stomatitis virus (VSV) and Herpes virus-1 (HSV-1). The impact of these IRIs was tested on the potency of the viral vectors using a plaque forming unit assay following room temperature storage, cryopreservation with successive freeze-thaw cycles and lyophilization. Viral potency after storage with all three conditions demonstrated that N-octyl-gluconamide (NOGlc) recovered the infectivity of shelf stored VV, 5.6 Log₁₀ PFU mL(-1) during 40 days, and HSV-1, 2.7 Log₁₀ PFU mL(-1) during 9 days. Carbon-linked antifreeze glycoprotein analogue ornithine-glycine-glycine-galactose (OGG-Gal) increases the recovery of VV and VSV more than 1 Log₁₀ PFU mL(-1) after 10 freeze-thaw cycles. In VSV, cryostorage with OGG-Gal maintains high infectivity and reduces temperature-induced aggregation of viral particles by 2 times that of the control. In total, OGG-Gal and NOGlc preserve virus potency during cryostorage. Remarkably, NOGlc has potential to eliminate the cold chain and permit room temperature storage of viral vectors.

Indexed as

CryopreservationHerpesvirus 1, HumanVaccinia virusVesiculovirusAntifreeze ProteinsCells, CulturedCryoprotective AgentsCrystallizationFreeze DryingGlycopeptidesHumansMicrobial ViabilityVaccines, Virus-Like ParticleAntifreeze ProteinsCryoprotective AgentsGlycopeptidesVaccines, Virus-Like Particle

Identifiers

PMID25078058
PMCPMC4116624
OpenAlexW1991333388

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.