ArticlePloS one2014
Real-time monitoring of protein conformational changes using a nano-mechanical sensor.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Unveiling the real-time behaviour of Staphylococcus aureus through nanomotion sensing.Scientific reports · 2025Article
- Review
- Toward a role for the acoustic field in cells interaction.Frontiers in systems neuroscience · 2025Article
- Accurate and rapid antibiotic susceptibility testing using a machine learning-assisted nanomotion technology platform.Nature communications · 2024Article
- Aclarubicin stimulates RNA polymerase II elongation at closely spaced divergent promoters.Science advances · 2023Article
- Review
- Nanomotion Spectroscopy as a New Approach to Characterize Bacterial Virulence.Microorganisms · 2021Article
- Nanomotion Detection-Based Rapid Antibiotic Susceptibility Testing.Antibiotics (Basel, Switzerland) · 2021Review
- A Review of the Effect of a Nanostructured Thin Film Formed by Titanium Carbide and Titanium Oxides Clustered around Carbon in Graphitic Form on Osseointegration.Nanomaterials (Basel, Switzerland) · 2020Review
- Single yeast cell nanomotions correlate with cellular activity.Science advances · 2020Article
- Silicon Microcantilever Sensors to Detect the Reversible Conformational Change of a Molecular Switch, Spiropyan.Sensors (Basel, Switzerland) · 2020Article
- A new tool to determine the cellular metabolic landscape: nanotechnology to the study of Friedreich's ataxia.Scientific reports · 2019Article
- Nanomotion detection based on atomic force microscopy cantilevers.Cell surface (Amsterdam, Netherlands) · 2019Review
- A Rapid Unraveling of the Activity and Antibiotic Susceptibility of Mycobacteria.Antimicrobial agents and chemotherapy · 2019Article
- Precision ultrasound sensing on a chip.Nature communications · 2019Article
- The role of oxidative stress in Friedreich's ataxia.FEBS letters · 2018Review
- Precise mass determination of single cell with cantilever-based microbiosensor system.PloS one · 2017Article
- Amyloid single-cell cytotoxicity assays by nanomotion detection.Cell death discovery · 2017Article
- Detecting nanoscale vibrations as signature of life.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteins can switch between different conformations in response to stimuli, such as pH or temperature variations, or to the binding of ligands. Such plasticity and its kinetics can have a crucial functional role, and their characterization has taken center stage in protein research. As an example, Topoisomerases are particularly interesting enzymes capable of managing tangled and supercoiled double-stranded DNA, thus facilitating many physiological processes. In this work, we describe the use of a cantilever-based nanomotion sensor to characterize the dynamics of human topoisomerase II (Topo II) enzymes and their response to different kinds of ligands, such as ATP, which enhance the conformational dynamics. The sensitivity and time resolution of this sensor allow determining quantitatively the correlation between the ATP concentration and the rate of Topo II conformational changes. Furthermore, we show how to rationalize the experimental results in a comprehensive model that takes into account both the physics of the cantilever and the dynamics of the ATPase cycle of the enzyme, shedding light on the kinetics of the process. Finally, we study the effect of aclarubicin, an anticancer drug, demonstrating that it affects directly the Topo II molecule inhibiting its conformational changes. These results pave the way to a new way of studying the intrinsic dynamics of proteins and of protein complexes allowing new applications ranging from fundamental proteomics to drug discovery and development and possibly to clinical practice.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.