ArticleJournal of visualized experiments : JoVE2014
ScanLag: high-throughput quantification of colony growth and lag time.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed.
- Population size modulates the transition from gene amplification-mediated heteroresistance to stable resistance.npj antimicrobials and resistance · 2026Article
- Iron acquisition strategies of the rock-inhabiting fungusApplied and environmental microbiology · 2026Article
- Structure-Activity Relationship Study of Antimicrobial Peptide with Cross-Kingdom Activity.Biochemistry · 2026Article
- Probiotic Viability Reconsidered: Integrating VBNC Resuscitation and Culture-Independent Methods for Accurate Probiotic Enumeration.Microorganisms · 2025Review
- Article
- Heteroresistance in Enterobacter cloacae complex caused by variation in transient gene amplification events.npj antimicrobials and resistance · 2025Article
- Article
- Relevance of charged and polar amino acids for functionality of membrane toxin TisB.Scientific reports · 2024Article
- Bacterial persisters: molecular mechanisms and therapeutic development.Signal transduction and targeted therapy · 2024Review
- Global Hfq-mediated RNA interactome of nitrogen starved Escherichia coli uncovers a conserved post-transcriptional regulatory axis required for optimal growth recovery.Nucleic acids research · 2024Article
- Microfluidic Giant Polymer Vesicles Equipped with Biopores for High-Throughput Screening of Bacteria.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- The metabolic slowdown caused by the deletion ofAntimicrobial agents and chemotherapy · 2024Article
- Bacterial colony size growth estimation by deep learning.BMC microbiology · 2023Article
- Article
- Use of the speckle imaging sub-pixel correlation analysis in revealing a mechanism of microbial colony growth.Scientific reports · 2023Article
- Serine-threonine phosphoregulation by PknB and Stp contributes to quiescence and antibiotic tolerance inScience signaling · 2023Article
- A Role for the RNA Polymerase Gene Specificity Factor σJournal of bacteriology · 2022Article
- Spatial segregation and cooperation in radially expanding microbial colonies under antibiotic stress.The ISME journal · 2021Article
- Clinically relevant mutations in core metabolic genes confer antibiotic resistance.Science (New York, N.Y.) · 2021Article
- LI Detector: a framework for sensitive colony-based screens regardless of the distribution of fitness effects.G3 (Bethesda, Md.) · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Growth dynamics are fundamental characteristics of microorganisms. Quantifying growth precisely is an important goal in microbiology. Growth dynamics are affected both by the doubling time of the microorganism and by any delay in growth upon transfer from one condition to another, the lag. The ScanLag method enables the characterization of these two independent properties at the level of colonies originating each from a single cell, generating a two-dimensional distribution of the lag time and of the growth time. In ScanLag, measurement of the time it takes for colonies on conventional nutrient agar plates to be detected is automated on an array of commercial scanners controlled by an in house application. Petri dishes are placed on the scanners, and the application acquires images periodically. Automated analysis of colony growth is then done by an application that returns the appearance time and growth rate of each colony. Other parameters, such as the shape, texture and color of the colony, can be extracted for multidimensional mapping of sub-populations of cells. Finally, the method enables the retrieval of rare variants with specific growth phenotypes for further characterization. The technique could be applied in bacteriology for the identification of long lag that can cause persistence to antibiotics, as well as a general low cost technique for phenotypic screens.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.