Evidence map›Paper›PMID 25077607›Full record

ArticlePloS one2014

Delivery of bioactive lipids from composite microgel-microsphere injectable scaffolds enhances stem cell recruitment and skeletal repair.

Anusuya Das, Daniel A Barker, Tiffany Wang, Cheryl M Lau, Yong Lin, Edward A Botchwey

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 31 citations in OpenAlex.

  1. Article
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  9. [Effects of FTY720-P on EphA2-EphrinA2 bidirectional signaling in osteoclasts].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2018
    Article
  10. [Effect of FTY720-P on the differentiation and maturation of MC3T3-E1 cells].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2018
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Anusuya DasDepartment of Orthopaedic Surgery, University of Virginia, Charlottesville, Virginia, United States of America; Department of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, United States of America.
Daniel A BarkerDepartment of Otolaryngology, University of Virginia, Charlottesville, Virginia, United States of America.
Tiffany WangDepartment of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia, United States of America.
Cheryl M LauDepartment of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia, United States of America.
Yong LinDepartment of Biomedical Engineering, University of Virginia, Charlottesville, Virginia, United States of America.
Edward A BotchweyDepartment of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia, United States of America.
Georgia Institute of Technology · USUniversity of Virginia · US

Funding

Phospholipid Growth Factors for Therapeutic Arteriogenesis and Tissue EngineeringR01AR056445 · NIAMS · UNIVERSITY OF VIRGINIA · PI BOTCHWEY, EDWARD A. · 2009 to 2014
$1.8M
Therapeutic S1P Drug Targets for Cranial Bone RepairR01DE019935 · NIDCR · UNIVERSITY OF VIRGINIA · PI BOTCHWEY, EDWARD A. · 2009 to 2013
$1.8M
NIAMS NIH HHS R01 AR056445NIDCR NIH HHS R01 DE019935
6 · The paper itself

Abstract

In this study, a microgel composed of chitosan and inorganic phosphates was used to deliver poly(lactic-co-glycolic acid) (PLAGA) microspheres loaded with sphingolipid growth factor FTY720 to critical size cranial defects in Sprague Dawley rats. We show that sustained release of FTY720 from injected microspheres used alone or in combination with recombinant human bone morphogenic protein-2 (rhBMP2) improves defect vascularization and bone formation in the presence and absence of rhBMP2 as evaluated by quantitative microCT and histological measurements. Moreover, sustained delivery of FTY720 from PLAGA and local targeting of sphingosine 1-phosphate (S1P) receptors reduces CD45+ inflammatory cell infiltration, promotes endogenous recruitment of CD29+CD90+ bone progenitor cells and enhances the efficacy of rhBMP2 from chitosan microgels. Companion in vitro studies suggest that selective activation of sphingosine receptor subtype-3 (S1P3) via FTY720 treatment induces smad-1 phosphorylation in bone-marrow stromal cells. Additionally, FTY720 enhances stromal cell-derived factor-1 (SDF-1) mediated chemotaxis of CD90+CD11B-CD45- bone progenitor cells in vitro after stimulation with rhBMP2. We believe that use of such small molecule delivery formulations to recruit endogenous bone progenitors may be an attractive alternative to exogenous cell-based therapy.

Indexed as

Bone RegenerationGelsMicrospheresTissue ScaffoldsAbnormalities, MultipleAnimalsCataractCell DifferentiationCell MovementCorneaFemaleFingolimod HydrochlorideHypogonadismIntellectual DisabilityLipidsMiceFingolimod HydrochlorideGelsLipidsPropylene GlycolsSphingosine

Identifiers

PMID25077607
PMCPMC4117484
OpenAlexW2002043903

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.