ArticleJournal of agricultural and food chemistry2014
Enhanced sensitive immunoassay: noncompetitive phage anti-immune complex assay for the determination of malachite green and leucomalachite green.
Article in Journal of agricultural and food chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- High SERS performance of functionalized carbon dots in the detection of dye contaminants.Journal of advanced research · 2025Article
- Review
- Dummy Template-Based Molecularly Imprinted Membrane Coating for Rapid Analysis of Malachite Green and Its Metabolic Intermediates in Shrimp and Fish.Molecules (Basel, Switzerland) · 2022Article
- Pretreatment Methods for Human Nasopharyngeal Swabs to Increase the Signal to Noise Ratio of High Sensitivity Immunoassays.ACS measurement science au · 2022Article
- Fluorescence detection of malachite green in fish based on aptamer and SYBR Green I.Journal of food and drug analysis · 2022Article
- Recombinant Peptide Mimetic NanoLuc Tracer for Sensitive Immunodetection of Mycophenolic Acid.Analytical chemistry · 2021Article
- Development of a sensitive non-competitive immunoassay via immunocomplex binding peptide for the determination of ethyl carbamate in wine samples.Journal of hazardous materials · 2021Article
- Detection of Malachite Green in Water Using Edge Excited Label Free Fluorescent Probe NCQDs.Journal of fluorescence · 2020Article
- Recyclable magnetic carbonaceous porous composites derived from MIL-100(Fe) for superior adsorption and removal of malachite green from aqueous solution.RSC advances · 2019Article
- Competitive and noncompetitive phage immunoassays for the determination of benzothiostrobin.Analytica chimica acta · 2015Article
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12 authors.
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No grant is acknowledged in the PubMed record.
Abstract
To develop a more sensitive immunoassay for malachite green (MG) and leucomalachite green (LMG), we identified the immunocomplex binding phage-borne peptides for use in the noncompetitive phage anti-immunocomplex assay (PHAIA). An anti-LMG monoclonal antibody (mAb) was used to select immunocomplex binding peptides from a circular random eight-amino-acid phage-displayed library. After three rounds of panning-elution, five peptides that bound the LMG-mAb immunocomplex were obtained. One of the phage-borne peptide clones that resulted in an assay with the highest sensitivity was chosen for further research. The concentration of LMG producing 50% of the saturated signal and the limit of detection of the assay were 7.02 and 0.55 ng/mL, respectively, with a linear range of 1.35 to 21.56 ng/mL. The PHAIA based on the same antibody was 16 times more sensitive compared to the competitive immunoassay. PHAIA was used to analyze LMG, MG, and two mixtures of spiked fish samples, with validation by high-performance liquid chromatography (HPLC) with fluorescence detector. Results showed a good correlation (R(2)LMG = 0.9841; R(2)MG = 0.993; R(2)Mixture = 0.9903) between the data of PHAIA and HPLC, thus the assay was an efficient method for monitoring food safety.
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