ArticleThe Journal of experimental medicine1989
Evolution of antibody structure during the immune response. The differentiative potential of a single B lymphocyte.
Article in The Journal of experimental medicine, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 70 citations in OpenAlex.
- Two Distinct Pathways in Mice Generate Antinuclear Antigen-Reactive B Cell Repertoires.Frontiers in immunology · 2018Article
- Large-scale sequence and structural comparisons of human naive and antigen-experienced antibody repertoires.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Influence of B cell antigen receptor expression level on pathways of B cell tolerance induction.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Silent development of memory progenitor B cells.Journal of immunology (Baltimore, Md. : 1950) · 2007Article
- Novel analysis of clonal diversification in blood B cell and bone marrow plasma cell clones in immunoglobulin light chain amyloidosis.Journal of clinical immunology · 2007Article
- A role for RAD51 in the generation of immunoglobulin gene diversity in rabbits.Journal of immunology (Baltimore, Md. : 1950) · 1999Article
- Homing behaviour of the malignant cell clone in multiple myeloma.Medical oncology (Northwood, London, England) · 1998Review
- The promotion of V region hypermutation.The Journal of experimental medicine · 1997Article
- The transcriptional promoter regulates hypermutation of the antibody heavy chain locus.The Journal of experimental medicine · 1997Article
- Tracing the development of single memory-lineage B cells in a highly defined immune response.The Journal of experimental medicine · 1996Article
- Altering the antibody repertoire via transgene homologous recombination: evidence for global and clone-autonomous regulation of antigen-driven B cell differentiation.The Journal of experimental medicine · 1995Article
- The CDR1 sequences of a major proportion of human germline Ig VH genes are inherently susceptible to amino acid replacement.Immunology today · 1994Review
- Article
- Somatic generation of hybrid antibody H chain genes in transgenic mice via interchromosomal gene conversion.The Journal of experimental medicine · 1994Article
- Structural analysis of the VH-D-JH segments of human polyreactive IgG mAb. Evidence for somatic selection.Journal of immunology (Baltimore, Md. : 1950) · 1993Article
- Isolation of germinal centerlike events from human spleen RNA. Somatic hypermutation of a clonally related VH6DJH rearrangement expressed with IgM, IgG, and IgA.The Journal of clinical investigation · 1993Article
- Hypermutation is observed only in antibody H chain V region transgenes that have recombined with endogenous immunoglobulin H DNA: implications for the location of cis-acting elements required for somatic mutation.The Journal of experimental medicine · 1993Article
- Molecular evolution of the human immunoglobulin E response: high incidence of shared mutations and clonal relatedness among epsilon VH5 transcripts from three unrelated patients with atopic dermatitis.The Journal of experimental medicine · 1993Article
- Somatic recombination of heavy chain variable region transgenes with the endogenous immunoglobulin heavy chain locus in mice.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Sequencing heavy- and light-chain variable genes of single B-hybridoma cells by total enzymatic amplification.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Changes in the structure and function of antibodies occur during the course of an immune response due to variable (V) region gene somatic mutation and isotype switch recombination. While the end products of both these processes are now well documented, their mechanisms, timing, and regulation during clonal expansion remain unclear. Here I describe the characterization of antibodies expressed by a large number of hybridomas derived from single B cell clones at an intermediate stage of an immune response. These data provide new insights into the mechanism, relative timing, and potential of V gene mutation and isotype switching. The data suggest that somatic mutation and isotype switching are completely independent processes that may, but need not, occur simultaneously during clonal expansion. In addition, the results of this analysis demonstrate that individual B cell clones are far more efficient than previously imagined at generating and fixing particular V region somatic mutations that result in increased affinity for the eliciting epitope. Models to account for this high efficiency are discussed. Taken together with previous data, the results of this analysis also suggest that the "somatic evolution" of V region structure to a single epitope takes place in two stages; the first in which particular mutations are sustained and fixed by antigen selection in the CDR regions of the V region genes expressed in a clone over a short period of clonal expansion, and the second in which these selected CDR mutations are maintained in the growing clone, deleterious mutations are lost, and selectively neutral mutations accumulate throughout the length of V genes over long periods of clonal expansion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.