ArticlePloS one2014
Mechanochemical phosphorylation and solubilisation of β-D-glucan from yeast Saccharomyces cerevisiae and its biological activities.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- β-glucan nanoparticles alleviate acute asthma by suppressing ferroptosis and DNA damage in mice.Apoptosis : an international journal on programmed cell death · 2025Article
- β-Glucan fragmentation by microfluidization and TNF-α-immunostimulating activity of fragmented β-glucans.Heliyon · 2024Article
- An important polysaccharide from fermentum.Food chemistry: X · 2022Review
- FT-IR Method Limitations for β-Glucan Analysis.Molecules (Basel, Switzerland) · 2022Article
- Characterization of β-Glucan-Peanut Protein Isolate/Soy Protein Isolate Conjugates and Their Application on Low-Fat Sausage.Molecules (Basel, Switzerland) · 2022Article
- Sustainable Production of Reduced Phosphorus Compounds: Mechanochemical Hydride Phosphorylation Using Condensed Phosphates as a Route to Phosphite.ACS central science · 2022Article
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Authors and funding
3 authors.
Funding
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Abstract
To obtain a water-soluble β-D-glucan derivative cleanly and conveniently, a highly efficient mechanochemical method, planetary ball milling, was used to phosphorylate β-D-glucan isolated from yeast Saccharomyces cerevisiae in solid state. Soluble β-D-glucan phosphate (GP) with a high degree of substitution (0.77-2.09) and an apparent PEAK molecular weight of 6.6-10.0 kDa was produced when β-D-glucan was co-milled with sodium hexametaphosphate at 139.5-186.0 rad/s for 12-20 min. The energy transferred was 3.03-11.98 KJ/g. The phosphorylation of GPs was demonstrated by Fourier transform infrared spectroscopy and 13C and 31P Nuclear magnetic resonance spectroscopy. Three GP products with different degree of substitution (DS) and degree of polymerisation (DP) were able to upregulate the functional events mediated by activated murine macrophage RAW264.7 cells, among which GP-2 with a DS of 1.24 and DP of 30.5 exerted the highest immunostimulating activity. Our results indicate that mechanochemical processing is an efficient method for preparing water-soluble and biologically active GP with high DS.
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