Evidence map›Paper›PMID 25074804›Full record

ArticleJournal of cell science2014

Coronin-1C and RCC2 guide mesenchymal migration by trafficking Rac1 and controlling GEF exposure.

Rosalind C Williamson, Christopher A M Cowell, Christina L Hammond, Dylan J M Bergen, James A Roper, Yi Feng, Thomas C S Rendall, Paul R Race, Mark D Bass

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cell science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
1.1field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 45 citations in OpenAlex.

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  12. Coronin 2B Regulates Neuronal Migration via Rac1-Dependent Multipolar-Bipolar Transition.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2023
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Rosalind C WilliamsonSchool of Biochemistry, University of Bristol, University Walk, Bristol BS8 1TD, UK.
Christopher A M CowellSchool of Biochemistry, University of Bristol, University Walk, Bristol BS8 1TD, UK.
Christina L HammondSchool of Biochemistry, University of Bristol, University Walk, Bristol BS8 1TD, UK.
Dylan J M BergenSchool of Biochemistry, University of Bristol, University Walk, Bristol BS8 1TD, UK.
James A RoperSchool of Biochemistry, University of Bristol, University Walk, Bristol BS8 1TD, UK.
Yi FengSchool of Biochemistry, University of Bristol, University Walk, Bristol BS8 1TD, UK.
Thomas C S RendallDepartment of Engineering, University of Bristol, University Walk, Bristol BS8 1TD, UK.
Paul R RaceSchool of Biochemistry, University of Bristol, University Walk, Bristol BS8 1TD, UK.
Mark D BassSchool of Biochemistry, University of Bristol, University Walk, Bristol BS8 1TD, UK mark.bass@bristol.ac.uk.
University of Bristol · GB

Funding

Wellcome Trust 088419Wellcome Trust 100104
6 · The paper itself

Abstract

Sustained forward migration through a fibrillar extracellular matrix requires localization of protrusive signals. Contact with fibronectin at the tip of a cell protrusion activates Rac1, and for linear migration it is necessary to dampen Rac1 activity in off-axial positions and redistribute Rac1 from non-protrusive membrane to the leading edge. Here, we identify interactions between coronin-1C (Coro1C), RCC2 and Rac1 that focus active Rac1 to a single protrusion. Coro1C mediates release of inactive Rac1 from non-protrusive membrane and is necessary for Rac1 redistribution to a protrusive tip and fibronectin-dependent Rac1 activation. The second component, RCC2, attenuates Rac1 activation outside the protrusive tip by binding to the Rac1 switch regions and competitively inhibiting GEF action, thus preventing off-axial protrusion. Depletion of Coro1C or RCC2 by RNA interference causes loss of cell polarity that results in shunting migration in 1D or 3D culture systems. Furthermore, morpholinos against Coro1C or RCC2, or mutation of any of the binding sites in the Rac1-RCC2-Coro1C complex delays the arrival of neural crest derivatives at the correct location in developing zebrafish, demonstrating the crucial role in migration guidance in vivo.

Indexed as

AnimalsCell MovementChromosomal Proteins, Non-HistoneGene Knockdown TechniquesGuanine Nucleotide Exchange FactorsHumansMesodermMiceMicrofilament ProteinsNeural Crestrac1 GTP-Binding ProteinSignal TransductionZebrafishChromosomal Proteins, Non-Histonecoronin proteinsGuanine Nucleotide Exchange FactorsMicrofilament Proteinsrac1 GTP-Binding ProteinRAC1 protein, humanRCC2 protein, humanCoro1CCoronin-1CMigrationNeural crestRac1RCC2

Identifiers

PMID25074804
PMCPMC4179493
OpenAlexW1983337669

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.