ArticleTraffic (Copenhagen, Denmark)2014
Arabinogalactan glycosyltransferases target to a unique subcellular compartment that may function in unconventional secretion in plants.
Article in Traffic (Copenhagen, Denmark), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 45 citations in OpenAlex.
- Plant-Derived Vesicle-like Nanoparticles: Pioneering Sustainable and Effective Approaches for Tissue Repair and Regeneration.Biomolecules · 2025Review
- Interkingdom Communication via Extracellular Vesicles: Unraveling Plant and Pathogen Interactions and Its Potential for Next-Generation Crop Protection.Microorganisms · 2024Review
- Plant-derived nanovesicles: Further exploration of biomedical function and application potential.Acta pharmaceutica Sinica. B · 2023Review
- Proteomics of Salt Gland-Secreted Sap Indicates a Pivotal Role for Vesicle Transport and Energy Metabolism in Plant Salt Secretion.International journal of molecular sciences · 2022Article
- Necessity of rice resistance to planthoppers for OsEXO70H3 regulating SAMSL excretion and lignin deposition in cell walls.The New phytologist · 2022Article
- ZmCTLP1 is required for the maintenance of lipid homeostasis and the basal endosperm transfer layer in maize kernels.The New phytologist · 2021Article
- Plant extracellular vesicles: Trojan horses of cross-kingdom warfare.FASEB bioAdvances · 2021Review
- Ride to cell wall: Arabidopsis XTH11, XTH29 and XTH33 exhibit different secretion pathways and responses to heat and drought stress.The Plant journal : for cell and molecular biology · 2021Article
- Message in a Bubble: Shuttling Small RNAs and Proteins Between Cells and Interacting Organisms Using Extracellular Vesicles.Annual review of plant biology · 2021Review
- The ArathEULS3 Lectin Ends up in Stress Granules and Can Follow an Unconventional Route for Secretion.International journal of molecular sciences · 2020Article
- Three Decades of Advances in Arabinogalactan-Protein Biosynthesis.Frontiers in plant science · 2020Review
- On the Potential Function of Type II ArabinogalactanFrontiers in plant science · 2020Review
- Engineering 'designer' glycomodules for boosting recombinant protein secretion in tobacco hairy root culture and studying hydroxyproline-O-glycosylation process in plants.Plant biotechnology journal · 2019Article
- A Hybrid Approach Enabling Large-Scale Glycomic Analysis of Post-Golgi Vesicles Reveals a Transport Route for Polysaccharides.The Plant cell · 2019Article
- The Multiple Facets of Plant-Fungal Interactions Revealed Through Plant and Fungal Secretomics.Frontiers in plant science · 2019Review
- Extracellular vesicles: a missing component in plant cell wall remodeling.Journal of experimental botany · 2018Article
- A broadly conserved NERD genetically interacts with the exocyst to affect root growth and cell expansion.Journal of experimental botany · 2018Article
- Post-Golgi Trafficking and Transport of Cell Wall Components.Frontiers in plant science · 2018Review
- Unconventional Transport Routes of Soluble and Membrane Proteins and Their Role in Developmental Biology.International journal of molecular sciences · 2017Article
- The plant secretory pathway seen through the lens of the cell wall.Protoplasma · 2017Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report that fluorescently tagged arabinogalactan glycosyltransferases target not only the Golgi apparatus but also uncharacterized smaller compartments when transiently expressed in Nicotiana benthamiana. Approximately 80% of AtGALT31A [Arabidopsis thaliana galactosyltransferase from family 31 (At1g32930)] was found in the small compartments, of which, 45 and 40% of AtGALT29A [Arabidopsis thaliana galactosyltransferase from family 29 (At1g08280)] and AtGlcAT14A [Arabidopsis thaliana glucuronosyltransferase from family 14 (At5g39990)] colocalized with AtGALT31A, respectively; in contrast, N-glycosylation enzymes rarely colocalized (3-18%), implicating a role of the small compartments in a part of arabinogalactan (O-glycan) biosynthesis rather than N-glycan processing. The dual localization of AtGALT31A was also observed for fluorescently tagged AtGALT31A stably expressed in an Arabidopsis atgalt31a mutant background. Further, site-directed mutagenesis of a phosphorylation site of AtGALT29A (Y144) increased the frequency of the protein being targeted to the AtGALT31A-localized small compartments, suggesting a role of Y144 in subcellular targeting. The AtGALT31A localized to the small compartments were colocalized with neither SYP61 (syntaxin of plants 61), a marker for trans-Golgi network (TGN), nor FM4-64-stained endosomes. However, 41% colocalized with EXO70E2 (Arabidopsis thaliana exocyst protein Exo70 homolog 2), a marker for exocyst-positive organelles, and least affected by Brefeldin A and Wortmannin. Taken together, AtGALT31A localized to small compartments that are distinct from the Golgi apparatus, the SYP61-localized TGN, FM4-64-stained endosomes and Wortmannin-vacuolated prevacuolar compartments, but may be part of an unconventional protein secretory pathway represented by EXO70E2 in plants.
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