ArticleNucleic acids research2014
3'LIFE: a functional assay to detect miRNA targets in high-throughput.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 52 citations in OpenAlex.
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- The miRNA-target interactions: An underestimated intricacy.Nucleic acids research · 2024Review
- Exosomal Circular Ribonucleic Acid-Microribonucleic Acid Expression Profile from Plasma in Alzheimer's Disease Patients by Bioinformatics and Integrative Analysis.The Eurasian journal of medicine · 2023Article
- miR-204 suppresses uveal melanoma cell migration and invasion through negative regulation of RAB22A.Functional & integrative genomics · 2023Article
- Review
- High-Throughput miRFluR Platform Identifies miRNA Regulating B3GLCT That Predict Peters' Plus Syndrome Phenotype, Supporting the miRNA Proxy Hypothesis.ACS chemical biology · 2021Article
- ceRNA Network Regulation of TGF-β, WNT, FOXO, Hedgehog Pathways in the Pharynx ofInternational journal of molecular sciences · 2021Article
- Validation of human microRNA target pathways enables evaluation of target prediction tools.Nucleic acids research · 2021Article
- CircFAM120B Blocks the Development of Colorectal Cancer by Activating TGF-Beta Receptor II Expression via Targeting miR-645.Frontiers in cell and developmental biology · 2021Article
- MicroRNA Expression Profiling, Target Identification, and Validation in Chondrocytes.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The tRNA pseudouridine synthase TruB1 regulates the maturation of let-7 miRNA.The EMBO journal · 2020Article
- Sweet Control: MicroRNA Regulation of the Glycome.Biochemistry · 2020Review
- Target prediction and validation of microRNAs expressed from FSHR and aromatase genes in human ovarian granulosa cells.Scientific reports · 2020Article
- MiR-135-5p inhibits TGF-β-induced epithelial-mesenchymal transition and metastasis by targeting SMAD3 in breast cancer.Journal of Cancer · 2020Article
- MicroRNA‑133b targets TGFβ receptor I to inhibit TGF‑β‑induced epithelial‑to‑mesenchymal transition and metastasis by suppressing the TGF‑β/SMAD pathway in breast cancer.International journal of oncology · 2019Article
- ALG-1 Influences Accurate mRNA Splicing Patterns in theGenetics · 2019Article
- Improved TGIRT-seq methods for comprehensive transcriptome profiling with decreased adapter dimer formation and bias correction.Scientific reports · 2019Article
- miR‑122 promotes proliferation and invasion of clear cell renal cell carcinoma by suppressing Forkhead box O3.International journal of oncology · 2019Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
MicroRNAs (miRNAs) are short non-coding RNAs that regulate gene output at the post-transcriptional level by targeting degenerate elements primarily in 3'untranslated regions (3'UTRs) of mRNAs. Individual miRNAs can regulate networks of hundreds of genes, yet for the majority of miRNAs few, if any, targets are known. Misexpression of miRNAs is also a major contributor to cancer progression, thus there is a critical need to validate miRNA targets in high-throughput to understand miRNAs' contribution to tumorigenesis. Here we introduce a novel high-throughput assay to detect miRNA targets in 3'UTRs, called Luminescent Identification of Functional Elements in 3'UTRs (3'LIFE). We demonstrate the feasibility of 3'LIFE using a data set of 275 human 3'UTRs and two cancer-relevant miRNAs, let-7c and miR-10b, and compare our results to alternative methods to detect miRNA targets throughout the genome. We identify a large number of novel gene targets for these miRNAs, with only 32% of hits being bioinformatically predicted and 27% directed by non-canonical interactions. Functional analysis of target genes reveals consistent roles for each miRNA as either a tumor suppressor (let-7c) or oncogenic miRNA (miR-10b), and preferentially target multiple genes within regulatory networks, suggesting 3'LIFE is a rapid and sensitive method to detect miRNA targets in high-throughput.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.