ArticleNucleic acids research2014
Broad-host-range vector system for synthetic biology and biotechnology in cyanobacteria.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers.
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Who cites it
103 citing papers in PubMed, 191 citations in OpenAlex.
- ModifiedMicroorganisms · 2026Article
- Removal of Photosystem I Subunit PsaE Leads to Photosynthetic Hydrogen Production in a Cyanobacterial PsaF-HoxYH Fusion Strain.Plant, cell & environment · 2026Article
- Performance of the pBHR1 mobilization protein MobV and its role in stable plasmid expression inMicrobiology spectrum · 2026Article
- A set of constitutive promoters with graded strengths for gene expression in diverse cyanobacterial strains.Applied and environmental microbiology · 2026Article
- Canonical transcription termination mechanisms explain a minority of operons in cyanobacteria.mSystems · 2026Article
- Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression.Current issues in molecular biology · 2026Review
- Synergy and antagonism in a genome-scale model of metabolic hijacking by bacteriophages.Science advances · 2026Article
- A set of constitutive promoters with graded strengths for gene expression in diverse cyanobacterial strains.bioRxiv : the preprint server for biology · 2026Article
- Harnessing engineered cyanobacteria for next-generation therapeutics.Archives of microbiology · 2026Review
- MALDI-FISH for Colocalization of Brominated Metabolites andJournal of natural products · 2026Article
- CyanoConstruct: simple platform for cyanobacterial expression construct assembly and translational tuning.BMC biotechnology · 2026Article
- Circadian clock proteins KaiB and Rbp2 ofMicrobiology spectrum · 2026Article
- Prokaryotic Molecular Defense Mechanisms and Their Potential Applications in Cancer Biology: A Special Consideration for Cyanobacterial Systems.Current issues in molecular biology · 2026Review
- Canonical transcription termination mechanisms explain a minority of operons in cyanobacteria.bioRxiv : the preprint server for biology · 2025Article
- Broad-Host-Range Synthetic Biology: Rethinking Microbial Chassis as a Design Variable.ACS synthetic biology · 2025Review
- Boosting energy metabolism and biosynthesis in diverse organisms by a common bacterial salvage lipoylation protein.Nature communications · 2025Article
- Establishing a Malonyl-CoA Biosensor for the Two Model CyanobacteriaACS synthetic biology · 2025Article
- Development of a CRISPR activation system for targeted gene upregulation in Synechocystis sp. PCC 6803.Communications biology · 2025Article
- dCas12a-mediated CRISPR interference for multiplex gene repression in cyanobacteria for enhanced isobutanol and 3-methyl-1-butanol production.Microbial cell factories · 2025Article
- Evaluation of vectors for gene expression inApplied and environmental microbiology · 2025Article
43 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
Inspired by the developments of synthetic biology and the need for improved genetic tools to exploit cyanobacteria for the production of renewable bioproducts, we developed a versatile platform for the construction of broad-host-range vector systems. This platform includes the following features: (i) an efficient assembly strategy in which modules released from 3 to 4 donor plasmids or produced by polymerase chain reaction are assembled by isothermal assembly guided by short GC-rich overlap sequences. (ii) A growing library of molecular devices categorized in three major groups: (a) replication and chromosomal integration; (b) antibiotic resistance; (c) functional modules. These modules can be assembled in different combinations to construct a variety of autonomously replicating plasmids and suicide plasmids for gene knockout and knockin. (iii) A web service, the CYANO-VECTOR assembly portal, which was built to organize the various modules, facilitate the in silico construction of plasmids, and encourage the use of this system. This work also resulted in the construction of an improved broad-host-range replicon derived from RSF1010, which replicates in several phylogenetically distinct strains including a new experimental model strain Synechocystis sp. WHSyn, and the characterization of nine antibiotic cassettes, four reporter genes, four promoters, and a ribozyme-based insulator in several diverse cyanobacterial strains.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.