Evidence map›Paper›PMID 25072505›Full record

ArticlePloS one2014

Sonic hedgehog-Gli1 signaling pathway regulates the epithelial mesenchymal transition (EMT) by mediating a new target gene, S100A4, in pancreatic cancer cells.

Xuanfu Xu, Bin Su, Chuangao Xie, Shumei Wei, Yingqun Zhou, Hua Liu, Weiqi Dai, Ping Cheng, Fan Wang, Xiaorong Xu and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.

0numbers the graph read from it
0cells of the map it votes in
56citing papers in PubMed
5.1field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

56 citing papers in PubMed, 84 citations in OpenAlex.

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  6. Uterine carcinosarcoma: Unraveling the role of epithelial-to-mesenchymal transition in progression and therapeutic potential.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024
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  18. Effect of shRNA-mediated regulation of S100A4 gene expression on proliferation and apoptosis of KLE endometrial cancer cells.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2021
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Xuanfu XuDepartment of Gastroenterology, the Tenth People's Hospital of Shanghai, Tongji University, Shanghai, China.
Bin SuDepartment of Endocrinology, the Tenth People's Hospital of Shanghai, Tongji University, Shanghai, China.
Chuangao XieDepartment of Gastroenterology, the Second Hospital of Zhejiang University, Hangzhou, Zhejiang Province, China.
Shumei WeiDepartment of Gastroenterology, the Second Hospital of Zhejiang University, Hangzhou, Zhejiang Province, China.
Yingqun ZhouDepartment of Gastroenterology, the Tenth People's Hospital of Shanghai, Tongji University, Shanghai, China.
Hua LiuDepartment of Gastroenterology, the Tenth People's Hospital of Shanghai, Tongji University, Shanghai, China.
Weiqi DaiDepartment of Gastroenterology, the Tenth People's Hospital of Shanghai, Tongji University, Shanghai, China.
Ping ChengDepartment of Gastroenterology, the Tenth People's Hospital of Shanghai, Tongji University, Shanghai, China.
Fan WangDepartment of Gastroenterology, the Tenth People's Hospital of Shanghai, Tongji University, Shanghai, China.
Xiaorong XuDepartment of Gastroenterology, the Tenth People's Hospital of Shanghai, Tongji University, Shanghai, China.
Chuanyong GuoDepartment of Gastroenterology, the Tenth People's Hospital of Shanghai, Tongji University, Shanghai, China.
Shanghai Tenth People's Hospital · CNTongji University · CNSecond Affiliated Hospital of Zhejiang University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimsThe hedgehog signaling pathway plays an important role in EMT of pancreatic cancer cells, but the precise mechanisms remain elusive. Because S100A4 as a key EMT moleculer marker was found to be upregulated upon Gli1 in pancreatic cancer cells, we focused on the relationship between Shh-Gli1 signals and S100 genes family.

methodsOn the base of cDNA microarray data, we investigated regulating mechanism of Gli1 to some members of S100A genes family in pancreatic cancer cell lines firstly. Then, the regulation of Gli1 to S100A4 gene was studied by molecular biology assays and the pro-metastasis effection of Gli1-dependent S100A4 was investigated in vitro. Finally, the expressions of Shh, Gli1, S100A4 and E-cadherin in pancreatic cancer tissues were studied by using immunohistochemistry assays.

resultsFive members of the S100 genes family, S100A2, S100A4, S100A6, S100A11, and S100A14 were found to be downregulated significantly upon Gli1 knockdown. Gli1 enhancer prediction combining with in vitro data demonstrated that Gli1 primarily regulates S100A family members via cis-acting elements. Indeed, the data indicate S100A4 and vimentin genes were upregulated significantly by Shh/Gli1-expression increasing and E-cadherin was significantly reduced at the same time. Migration of PC cells was increased significantly in a dose-dependent manner of Gli1 expression (P<0.05) and siS100A4 significantly reversed the response of PC cells induced by L-Shh transduction (P<0.01).

conclusionOur data establish a novel connection between Shh-Gli1 signaling and S100A4 regulation, which imply that S100A4 might be one of the key factors in EMT mediated by Shh-Gli1 signaling in pancreatic cancer.

Indexed as

AdultAgedBase SequenceBinding SitesCadherinsCell Line, TumorCell MovementDown-RegulationEpithelial-Mesenchymal TransitionFemaleHedgehog ProteinsHT29 CellsHumansMaleMiddle AgedPancreatic NeoplasmsCadherinsGLI1 protein, humanHedgehog ProteinsRNA, Small InterferingS100A4 protein, humanS100 Calcium-Binding Protein A4S100 ProteinsTranscription FactorsVimentinZinc Finger Protein GLI1

Identifiers

PMID25072505
PMCPMC4114558
OpenAlexW2048000049

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.