ArticlePloS one2014
Argonaute 2 in cell-secreted microvesicles guides the function of secreted miRNAs in recipient cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 45 citations in OpenAlex.
- siRNA-AGO2 complex inhibits bacterial gene translation: A promising therapeutic strategy for superbug infection.Cell reports. Medicine · 2025Article
- Extracellular Vesicles and Immunity: At the Crossroads of Cell Communication.International journal of molecular sciences · 2024Review
- The microRNA Lifecycle in Health and Cancer.Cancers · 2022Review
- Review
- Exosome Carrier Effects; Resistance to Digestion in Phagolysosomes May Assist Transfers to Targeted Cells; II Transfers of miRNAs Are Better Analyzed via Systems Approach as They Do Not Fit Conventional Reductionist Stoichiometric Concepts.International journal of molecular sciences · 2022Review
- Extracellular vesicles: General features and usefulness in diagnosis and therapeutic management of colorectal cancer.World journal of gastrointestinal oncology · 2021Review
- Diverse roles of EV-RNA in cancer progression.Seminars in cancer biology · 2021Review
- RNAs on the Go: Extracellular Transfer in Insects with Promising Prospects for Pest Management.Plants (Basel, Switzerland) · 2021Review
- Extracellular vesicle therapy for retinal diseases.Progress in retinal and eye research · 2020Review
- Challenges in Using Circulating Micro-RNAs as Biomarkers for Cardiovascular Diseases.International journal of molecular sciences · 2020Review
- Co-expression network analysis predicts a key role of microRNAs in the adaptation of the porcine skeletal muscle to nutrient supply.Journal of animal science and biotechnology · 2020Article
- The green peach aphid gut contains host plant microRNAs identified by comprehensive annotation of Brassica oleracea small RNA data.Scientific reports · 2019Article
- Contribution of Extracellular Vesicles in Rebuilding Injured Muscles.Frontiers in physiology · 2019Review
- Protein-based vehicles for biomimetic RNAi delivery.Journal of biological engineering · 2019Review
- Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Promote Neuroprotection in a Genetic DBA/2J Mouse Model of Glaucoma.Investigative ophthalmology & visual science · 2018Article
- miRNA-Mediated Interactions in and between Plants and Insects.International journal of molecular sciences · 2018Review
- Structurally modulated codelivery of siRNA and Argonaute 2 for enhanced RNA interference.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Promote Neuroprotection in Rodent Models of Glaucoma.Investigative ophthalmology & visual science · 2018Article
- microRNA in Cardiovascular Aging and Age-Related Cardiovascular Diseases.Frontiers in medicine · 2017Review
- Cell-free microRNAs in blood and other body fluids, as cancer biomarkers.Cell proliferation · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) secreted by cells into microvesicles (MVs) form a novel class of signal molecules that mediate intercellular communication. However, several fundamental aspects of secreted miRNAs remain unknown, particularly the mechanism that governs the function or fate of exogenous miRNAs in recipient cells. In the present study, we provide evidence indicating that Argonaute 2 (Ago2) plays a role in stabilizing miRNAs and facilitating the packaging of secreted miRNAs into MVs. More importantly, Ago2 in origin cell-secreted MVs (but not in recipient cells) directs the function of secreted miRNAs. First, Ago2 overexpression clearly increased the level of miR-16 in cells transfected with a miR-16 mimic by protecting the miRNAs from degradation in lysosomes. Second, Ago2 overexpression increased the level of miR-16 in cell-secreted MVs, suggesting that Ago2 may facilitate the packaging of secreted miRNAs into MVs. Third, exogenous miR-16 delivered by MVs within the origin cells significantly reduced the Bcl2 protein level in recipient cells, and miR-16 and Bcl2 mRNA were physically associated with exogenous HA-tagged Ago2 (HA-Ago2). Finally, the effect of MV-delivered miR-16 on the production of the Bcl2 protein in recipient cells was not abolished by knocking down Ago2 in the recipient cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.